异氟醚后处理对缺血缺氧性脑损伤新生大鼠学习策略运动功能及生长发育的影响  

Effects of Isoflurane Postconditioning on Learning Strategies,Motor Function and Growing Development of Hypoxic-ischemia Brain Injury in Neonatal Rats

作　　者：徐莹[1] 纪国余 王菲菲[1] 杨雅婷[1] 赵平[1] 

机构地区：[1]中国医科大学附属盛京医院麻醉二科,沈阳110004

出　　处：《中国医科大学学报》2014年第12期1066-1070,共5页Journal of China Medical University

基　　金：国家自然科学基金(81171782)

摘　　要：目的探讨异氟醚后处理对缺血缺氧性脑损伤(HIBI)新生大鼠学习策略、运动功能及生长发育的影响。方法新生SD大鼠105只,体质量12~16 g,将大鼠随机分为7组(n=15):假手术组、模型组、异氟醚组、苍术苷组、苍术苷+异氟醚组、环孢素组、环孢素+异氟醚组。各组均行左侧颈总动脉结扎术(假手术组不结扎),吸入8%O2+92%N2混合气体处理2 h,制备新生大鼠缺血缺氧性脑损伤(HIBI)模型。各组于建模(或假手术)后,侧脑室注射药物:苍术苷组及苍术苷+异氟醚组注射苍术苷;环孢素及环孢素+异氟醚组注射环孢素A;其余组注射生理盐水。在此基础上异氟醚组、苍术苷+异氟醚组及环孢素+异氟醚组予以1.5%异氟醚后处理30 min。于缺血缺氧后,21 d^28 d行悬吊实验,30 d^35 d行Morris水迷宫实验,水迷宫实验结束后测定大鼠体质量,评估各组大鼠的学习策略构成、运动功能及生长发育情况。结果与假手术组比较,其余组总学习型策略构成比小(P<0.05);与模型组比较,异氟醚组及两环孢素处理组总学习型策略构成比大(P<0.05),而两苍术苷处理组总学习型策略无统计学差异(P>0.05);与异氟醚组比较,两苍术苷处理组非学习型游泳策略构成比大(P<0.05),而两环孢素组总学习型策略无统计学差异(P>0.05)。各组大鼠悬吊实验时间、游泳速度、游泳路程以及不同阶段体质量无统计学差异(P>0.05)。结论异氟醚后处理对HIBI新生大鼠的运动功能及生长发育没有影响,但是可以改善HIBI新生大鼠的学习策略构成。Objective To investigate the effects of isoflurane postconditioning on learning strategies, motor function and growing development of hy- poxic-ischemia brain injury （HIBI） in neonatal rats. Methods A total of 105 Sprague- Dawley（SD） neonatal rats were randomly divided into 7 groups using the random number table method, sham surgery group, model group, isoflurane group, atractyloside group, atractyloside with isoflurane group, cyclosporine group and cyclosporine with isofluranc group. To establish HIBI models, each group underwent the left common carotid artery li- gation （sham group threading without ligation）, and inhaled 8% oxygen, 92% nitrogen mixed gas. After model was established （or sham surgery）, drug administration was performed through lateral ventricle ： atractyloside group and atractyloside with isoflurane postconditioning group were inject- ed atractyloside, cyclosporine group and cyclosporine with isoflurane postconditioning group were injected cyclosporine A, other groups were injected saline. On this basis, in soflurane postconditioning group, atractyloside with isoflurane postconditioning group and cyclosporine with isoflurane post- conditioning group, 1.5% isoflurane were inhaled continuously for 30 min. After brain hypoxia-ischemic brain injury, conducting tail suspension test on the 21st to 28th days, and Morris water maze test on the 30th to 35th days, and body weight of rats in each group was measured after water Morris maze test, learning strategies, motor function and growing development of each group rats were evaluated. Results Compared with the sham surgery group, the proportion of learning strategies was decreased in other groups （P 〈 0.05 ）. Compared with model group, the proportion of learning strate- gies were increased in isoflurane group, cyclosporine group and cyclosporine with isoflurane group （P 〈 0.05 ）. There were no significantly difference between atractyloside group and atractyloside with isoflurane group （P 〉 0.05 ）. Compared with isofl

关 键 词：异氟醚 后处理 缺血缺氧性脑损伤 水迷宫 生长发育 

分 类 号：R614.2[医药卫生—麻醉学]