MALDI-TOF-MS鉴定水稻细菌性叶鞘褐腐病菌的方法研究  

Identification of Pseudomonas fuscovaginae by MALDI-TOF-Mass spectrometry

作　　者：黄迎波[1] 周慧平[1] 莫瑾[1] 谭建锡[1] 彭梓[1] 朱金国[1] 唐连飞[1] 

机构地区：[1]湖南出入境检验检疫局,湖南长沙410004

出　　处：《植物检疫》2014年第6期54-58,共5页Plant Quarantine

基　　金：国家"十二五"科技支撑计划课题(2012BAC01B07);国家质检总局课题(2003IK067)

摘　　要：利用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术对3株水稻细菌性叶鞘褐腐病菌(Pseudomonas fuscovaginae)标准菌株进行蛋白质指纹图谱分析,将不同培养基、不同培养时间和不同样品处理方法等条件下所收集到的质谱峰进行比较,筛选出最佳实验方法,收集20张参考图谱与水稻常见病原细菌质谱峰进行比较,建立该病菌的蛋白质指纹图谱。结果显示用金氏B培养基分离纯化后经提取处理法进行MALDI-TOF-MS分析为最佳的实验方法。该方法对检测与鉴定水稻细菌性叶鞘褐腐病菌具有较高的灵敏度和重复性,且分析过程简单快速,有一定的应用价值。Matrix assisted laser desorption ionization time of flight （MALDI-TOF-MS） was used to analyze protein mass fingerprints of three standard strains of Pseudomonas fuscovaginae, Mass spectrum peak on different medium, different training time and different of sample treatment methods were compared to obtain the best experimental methods, collect 20 pieces of reference map and rice common pathogenic bacteria mass peak compared, to establish the germ protein fingerprint. Results show that Kim B medium purification after the extraction process for MALDI-TOF-MS analysis is the best method of the experiment. This method in the detection and identification of P.fuscovaginae has high sensitivity and repeatability, and the analysis process simply and quickly, and application of significance.

关 键 词：基质辅助激光解吸电离飞行时间质谱 蛋白质指纹图谱 水稻细菌性叶鞘褐腐病菌 鉴定 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]