黑麦草属DNA条形码鉴定技术研究  

Establishment of DNA barcode of common species of Lolium

作　　者：李阳[1] 吴申懋 陈沁[1] 

机构地区：[1]上海大学生命科学学院,上海200444

出　　处：《植物检疫》2014年第6期73-78,共6页Plant Quarantine

基　　金：国家质检总局项目(2013IK281);国家科技支撑计划(2012BAK11B03)

摘　　要：以毒麦、田毒麦、多花黑麦草、多年生黑麦草、硬直黑麦草、高羊茅与狗牙根等禾本科的7种植物为材料,拟采用DNA测序、特异性位点比对、种间遗传距离测定、建立系统树等分析候选DNA条形码psb A-trn H鉴别黑麦草属常见植物的能力。实验结果表明,以psb A-trn H为DNA条形码时,建立的系统发育树能较好区分毒麦和田毒麦与其他几种植物。psb A-trn H序列可以作为黑麦草属植物的潜在条形码。7 kinds of plants such as Lolium temulentum、Lolium temulentum L.var. arvense Bab.、Lolium multiflorum、Lolium perenne、Lolium rigidum、Festuca、Cynodon are used as materials, which belong to Poaceae, to analyze psbA-trnH candidate DNA barcode’s ability of identifying the common species of Lolium by DNA sequencing, specific loci comparisons and interspecific genetic distance measurement, establishing the system tree and other technical means. The results show that Lolium temulentum and Lolium temulentum L.var. arvense Bab. can be divided well by the established system tree when we use psbA-trnH sequence as a DNA barcode. psbA-trnH could be used as a potential DNA barcode to correctly identify the plants in Lolium.

关 键 词：DNA 条形码 黑麦草 毒麦 鉴定 

分 类 号：S543.6[农业科学—作物学]