苹果异形小卷蛾的分子鉴定研究  

Molecular identification of Thaumatotibia leucotreta(Lepidoptera, Tortricidae)

作　　者：余辛 刘军民 章柱 林晓红 刘伟 岳巧云 冯丽 

机构地区：[1]广州机场出入境检验检疫局,广东广州510410 [2]中山出入境检验检疫局

出　　处：《植物检疫》2014年第6期79-83,共5页Plant Quarantine

基　　金：广东检验检疫局科技计划项目﹙2014GDK87﹚;广东检验检疫局科技计划项目﹙2014GDK21﹚

摘　　要：苹果异形小卷蛾(Thaumatotibia leucotreta)是世界范围内重要的检疫性有害生物,对水果和农作物等生产具有重要影响,并影响国际贸易发展。本研究选用线粒体COI基因作为物种鉴定的条形码,结合Gen Bank数据库、BOLD数据库比对和系统进化树构建,对2014年1月广州白云国际机场口岸入境加纳牛油果中截获的卷蛾科昆虫进行分子鉴定。根据Gen Bank数据库比对分析、NJ方法构建的系统进化树分析以及BOLD数据库查询分析,最终鉴定该样品为苹果异形小卷蛾。认为基于DNA条形码的分子鉴定能快速、准确地鉴定苹果异形小卷蛾。Thaumatotibia leucotreta is a worldwide important quarantine pests, which has caused a great harm to fruit and crop production, and had impact on international trade. In this study, we amplified the COI fragment from the Tortricidae samples which were intercepted in avocados carried by a tourist from Garner at Guangzhou Baiyun Airport in January of 2014. We sequenced the fragments and blasted in the GenBank, Barcoding of Life Data Systems （BOLD）. A neighbor-joining tree was constructed. The intercepted insects were identified as Thaumatotibia leucotreta based on the DNA barcodes results. Our results demonstrate that, the DNA barcode could be used for quick and accurate identification of Thaumatotibia leucotreta.

关 键 词：苹果异形小卷蛾 DNA 条形码 COI 基因 分子鉴定 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]