Molecular Cytogenetic Characterization and Stem Rust Resistance of Five Wheat-Thinopyrum ponticum Partial Amphiploids  

作　　者：Qi Zheng Zhenling Lv Zhixia Niu Bin Li Hongwei Li Steven S.Xu Fangpu Han Zhensheng Li 

机构地区：[1]State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences [2]United States Department of Agriculture,Agricultural Research Service,Cereal Crops Research Unit

出　　处：《Journal of Genetics and Genomics》2014年第11期591-599,共9页遗传学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(No.31171539);the National High-Tech Research and Development Program of China(No.2011AA1001);the National Key Technology R&D Program of China(No.2013BAD05B01)

摘　　要：Partial amphiploids created by crossing common wheat （Triticum aestivum L.） and Thinopyrum ponticum （Podp.） Barkworth ＆ D. R. Dewey are important intermediates in wheat breeding because of their resistance to major wheat diseases. In this study, we examined the chromosome compositions of five Xiaoyan-series wheat-Th, ponticum partial amphiploids （Xiaoyan 68, Xiaoyan 693, Xiaoyan 784, Xiaoyan 7430, and Xiaoyan 7631） using GISH, multicolor-GISH, and multicolor-FISH. We found several chromosome changes in these lines. For example, wheat chromosomes 1B and 2B were added in Xiaoyan 68 and Xiaoyan 7430, respectively, while wheat chromosome 6B was eliminated from Xiaoyan 693 and Xiaoyan 7631. Chromosome rearrangements were also detected in these amphiploids, including an interspecific translocation involving chromosome 4D and some intergenomic translocations, such as A--B and A--D translocations, among wheat genomes. Analysis of the Th. ponticum chromosomes in the amphiploids showed that some lines shared the same alien chromosomes. We also evaluated these partial amphiploids for resistance to nine races of stem rust, including TTKSK （commonly known as Ug99）. Three lines, Xiaoyan 68, Xiaoyan 784, and Xiaoyan 7430, exhibited excellent resistance to all nine races, and could therefore be valuable sources of stem rust resistance in wheat breeding.Partial amphiploids created by crossing common wheat （Triticum aestivum L.） and Thinopyrum ponticum （Podp.） Barkworth ＆ D. R. Dewey are important intermediates in wheat breeding because of their resistance to major wheat diseases. In this study, we examined the chromosome compositions of five Xiaoyan-series wheat-Th, ponticum partial amphiploids （Xiaoyan 68, Xiaoyan 693, Xiaoyan 784, Xiaoyan 7430, and Xiaoyan 7631） using GISH, multicolor-GISH, and multicolor-FISH. We found several chromosome changes in these lines. For example, wheat chromosomes 1B and 2B were added in Xiaoyan 68 and Xiaoyan 7430, respectively, while wheat chromosome 6B was eliminated from Xiaoyan 693 and Xiaoyan 7631. Chromosome rearrangements were also detected in these amphiploids, including an interspecific translocation involving chromosome 4D and some intergenomic translocations, such as A--B and A--D translocations, among wheat genomes. Analysis of the Th. ponticum chromosomes in the amphiploids showed that some lines shared the same alien chromosomes. We also evaluated these partial amphiploids for resistance to nine races of stem rust, including TTKSK （commonly known as Ug99）. Three lines, Xiaoyan 68, Xiaoyan 784, and Xiaoyan 7430, exhibited excellent resistance to all nine races, and could therefore be valuable sources of stem rust resistance in wheat breeding.

关 键 词：Thinopyrum ponticum Triticum aestivum Partial amphiploid Multicolor-genomic in situ hybridization （mc-GISH） Multicolor-fluorescent insitu hybridization （me-FISH） Stem rust 

分 类 号：S512.1[农业科学—作物学] S512.103.5