极低频高压脉冲电场对萌发绿豆干旱伤害的缓解作用  

Alleviating effect of extremely low frequency high-voltage pulsed electric field on drought damage of geminating mung beans

作　　者：高宇[1] 习岗[1] 杨运经[2] 刘锴[1] 

机构地区：[1]西安理工大学应用物理系,陕西西安710048 [2]西北农林科技大学应用物理系,陕西杨凌712100

出　　处：《干旱地区农业研究》2014年第6期82-86,118,共6页Agricultural Research in the Arid Areas

基　　金：国家自然科学基金(50977079;51277151)

摘　　要：采用峰值场强为100 k V·m-1、脉宽80 ms、脉冲频率为1 Hz的极低频高压脉冲电场(ELF-PEF)处理-0.1 MPa的PEG-6000溶液中萌发的绿豆种子,研究了绿豆在萌发过程中鲜重、自发发光、延迟发光、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性的变化。结果表明,ELF-PEF对PEG胁迫下萌发的绿豆鲜重、自发发光和延迟发光积分强度促进作用分别达到了28.58%、78.35%和40.6%,说明ELF-PEF可以有效促进绿豆种子萌发过程中的生理代谢,缓解干旱胁迫对细胞的伤害作用。ELF-PEF通过提高PEG胁迫下萌发绿豆中SOD活性,降低MDA含量,缓解干旱胁迫对萌发绿豆的氧化损伤,ELF-PEF对细胞毛细吸水和代谢吸水的促进可能是其缓解干旱伤害的原因。Mung beans soaked in -0 .1MPa PEG solution were treated by extremely low frequency pulsed electric field （ELF-PEF）with peak electric field strength of 100 kV·m^-1 ,pulse width of 80 ms ,and pulse frequency of 1 Hz . Traits like fresh weight ,spontaneous luminescence ,delayed luminescence ,malondialdehyde （MDA ） and superoxide dis-mutase （SOD） activity during mung beans germination were investigated .The fresh weight ,spontaneous luminescence and delayed luminescence integral intensity in germinating mung beans were 28 .58% ,78 .35% and 40 .6% ,respec-tively under PEG stress ,indicating that ELF-PEF can accelerate the physiological metabolismand alleviate cells injury under drought stress . Although with increased SOD activity and simultaneously reducing MDA content , the oxidation damage caused by drought stress was alleviated by ELF-PEF in germinating mung beans .The promoting effect of ELF-PEF on capillary water absorption and metabolism water absorption in cells may be the cause of alleviating drought injury .

关 键 词：极低频高压脉冲电场 萌发绿豆 自发发光 延迟发光 丙二醛 超氧化物歧化酶 

分 类 号：S522.031[农业科学—作物学] S125