利用分子标记选择普通菜豆抗角斑病基因聚合体  

Molecular Marker-Assisted Selection for Pyramiding Angular Leaf Spot Resistance Genes in Common Bean(Phaseolus vulgaris L.)

作　　者：郝俊杰[1] 张晓艳[1] 万述伟[1] 李红卫[1] 赵爱鸿 孙吉禄[1] 王珍青[1] 

机构地区：[1]青岛市农业科学研究院,山东青岛266100 [2]青岛市农产品质检中心,山东青岛266000

出　　处：《作物杂志》2014年第6期27-31,共5页Crops

基　　金：现代农业产业技术体系专项资金(CARS-09);农业部"引进国际先进农业科学技术"项目[2012Z-52;2011-G1(3)-08];山东省农业良种工程(2013LZ01-01-02);公益性行业专项(201303007-3);青岛农科院博士基金项目

摘　　要：明确菜豆抗角斑病基因在中国普通菜豆中的分布,有助于改良普通菜豆抗病性和提高产量潜力。利用普通菜豆抗角斑病基因H13、NO2、EACA、AA19、BA16及MO2的特异PCR标记,对78份普通菜豆资源进行抗角斑病基因聚合体的选择,从中选择到含2个抗角斑病基因聚合体的菜豆资源10份,含3个抗角斑病基因聚合体的菜豆资源30份,含4个抗角斑病基因聚合体的菜豆资源34份,含5个抗角斑病基因聚合体的菜豆资源3份。该研究明确了78份参试菜豆资源所含的抗角斑病基因类型,筛选出了抗角斑病基因聚合体的菜豆资源。The PCR markers of the angular leaf spot resistance gene H13 ,NO2 ,E-ACA ,AA19 ,BA16 and MO2 were used to identify the genome DNA of 78 common bean accessions. The results indicated that 10 accessions had 2 pyramiding genes,30 accessions had 3 pyramiding genes, 34 accessions had 4 pyramiding genes and 3 accessions had 5 pyramiding genes. The types of angular leaf spot resistant genes in each of the 78 accessions had been identi- fied, and the accessions with pyramiding angular leaf spot resistance genes were selected.

关 键 词：普通菜豆 角斑病 抗病基因 聚合体 分子标记 

分 类 号：S643.1[农业科学—蔬菜学]