黄瓜绿斑驳病毒人工诱变方法的研究  

Study on the Artificial Mutagenesis Method for Cucumber Green Mottle Mosaic Virus

作　　者：钟敏[1] 赵绪生[1] 何乙坤 王亚南[1] 曹克强[1] 

机构地区：[1]河北农业大学植物保护学院,河北保定071001

出　　处：《北方园艺》2014年第24期108-111,共4页Northern Horticulture

基　　金：国家自然科学基金资助项目(31201487);河北省自然科学基金资助项目(C2013204058);"河北省青年拔尖人才计划"资助项目

摘　　要：以黄瓜绿斑驳病毒(Cucumber green mottle mosaic virus)野生株系为试材,采用高温处理、亚硝酸处理及复合处理对其进行人工诱变,比较3种诱变方法致弱效果。结果表明:3种方法均具有削弱病毒群体在苋色藜上枯斑扩展的能力,野生株系接种苋色藜后,直径小于1mm枯斑比率为1.99%,亚硝酸处理、高温处理、复合处理小枯斑比率分别为11.41%、12.87%、14.20%,复合处理致弱效果最好。该结果有助于快速有效的筛选CGMMV弱毒株,也将为弱毒疫苗的开发和致病相关基因功能的研究奠定基础。The wild isolate of cucumber green mottle mosaic virus was mutated by three artificial methods,including heat treatment,nitrous acid treatment and combination treatment,the attenuation effect of the three methods were compared.The results showed that all of the three treatments could attenuate virus to product smaller local lesion on Chenopodium amaranticolor.The attenuated rate of heat treatment,nitrous acid treatment and combination treatment were 11.41%,12.87%and 14.20%respectively.Chenopodium amaranticolor inoculated by wild strain was used as positive control,the attenuated rate of which was 1.99%.It presumed that the attenuation effect of combination treatment was the best.The result was useful to obtain CGMMV attenuated strain rapidly and effectively and set a good base for developing attenuated vaccine and studying the function of pathogenic genes in the future.

关 键 词：黄瓜绿斑驳病毒 人工诱变 弱毒株 

分 类 号：S642.2[农业科学—蔬菜学]