PiB对胶质瘤细胞增殖与侵袭能力的影响  

Pi B inhibit the proliferation and invasiveness of glioma cells

作　　者：倪升远 牛朝诗[1,2,3] 杨洋[1,2] 汪炎[1,2] 李仲颖[1,2] 贺虎[1,2] 李冬雪[1,2] 程传东[1,2] 

机构地区：[1]安徽医科大学附属省立医院神经外科,合肥230001 [2]安徽医科大学附属省立医院脑功能与脑疾病安徽省重点实验室 [3]安徽省脑立体定向神经外科研究所

出　　处：《中国微侵袭神经外科杂志》2014年第12期558-561,共4页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：国家自然科学基金项目(编号:81172407);安徽省重点实验室绩效考核项目(编号:1306c083028)

摘　　要：目的探讨PIN1抑制剂Pi B对U87胶质瘤细胞系增殖、迁移和侵袭能力的影响。方法人胶质瘤细胞系U87常规培养,取对数生长期细胞进行试验,以1.0μmol/L Pi B处理U87细胞48 h为实验组,未经任何处理的U87细胞作为对照组。RT-PCR和Western blotting检测PIN1基因的m RNA和蛋白表达。免疫荧光检测阳性细胞数。Transwell实验检测细胞迁移和侵袭能力的改变。MTT比色法检测Pi B对细胞增殖的抑制作用。结果与对照组相比,实验组细胞PIN1基因的m RNA和蛋白表达水平明显下调,PIN1阳性细胞数明显减少,细胞抑制率明显,且与药物剂量和作用时间成相关性,细胞迁移和侵袭能力下降,差异有统计学意义(P<0.05)。结论 Pi B可靶向抑制PIN1基因的m RNA和蛋白水平表达,进而抑制胶质瘤细胞增殖、迁移和侵袭能力。Objective To investigate the effect of PiB induced repression of PIN1 on the proliferation, migration and invasion of U87 glioblastoma cell line. Methods U87 glioblastoma cell lines were cultured, and the cells in the logarithmic phase were harvested for experiments. U87 glioblastoma cells were treated with 1.0 mol/L PiB for 48 h in the experimental group and the untreated U87 cells served as control group. The mRNA and protein expression of PIN1 gene were detected by RT-PCR and Western blotting. The positive cells were detected by immunofluorescence staining. Migration and invasion of U87 glioblastoma cells were detected by transwell migration and invasion assays. MTT assay was used to detect the inhibitory effect of PiB on proliferation of U87 glioblastoma cells. Results Compared with the control group, mRNA and protein expressions of PIN1 gene were significantly down-regulated in the experimental group cells. Immunofluorescence staining results showed that PINl-positive cells were significantly reduced in the experimental group. The MTT showed that the cell inhibition rate was increased obviously, and correlated with the drug dose and action time. Transwell test showed cell migration and invasion abilities were decreased obviously （P 〈 0.05）. Conclusions PiB can suppress the mRNA and protein expression of PIN1 gene, thereby inhibiting the proliferation, migration and invasion abilities of glioma cells.

关 键 词：神经胶质瘤 肽基脯氨酰异构酶1 酶抑制剂 增殖 

分 类 号：R739.41[医药卫生—肿瘤]