补肾助阳方对植物雌激素致勃起功能下降干预的实验研究  

Therapeutic efficacy of Bushengzhuyang Fang (Yangjing Capsule) on phytoestrogen-induced erectile dysfunction: An experimental study

作　　者：周玉春[1] 李天赋 孙志兴[1] 樊千[1] 刘涛[1] 金保方[1] 

机构地区：[1]南京中医药大学第一临床医学院,江苏南京210029 [2]南京军区南京总医院解放军临床检验医学研究所,江苏南京210002

出　　处：《中华男科学杂志》2014年第12期1103-1108,共6页National Journal of Andrology

基　　金：江苏省高校自然科学研究计划(12KJB360007)~~

摘　　要：目的:研究补肾助阳方(养精胶囊)对大鼠阴茎勃起功能的影响机制。方法:成年雄性SD大鼠56只,随机分为7组,分别为空白对照组、大豆黄酮组、十一酸睾酮组、西地那非组及养精胶囊(高/中/低)剂量组。空白对照组给予生理盐水灌胃,其余各组100 mg/(kg·d)大豆黄酮灌胃30d。随后各实验组在给予大豆黄酮灌胃的同时,养精胶囊(高/中/低)剂量组分别给予1.26、0.63、0.315 mg/k/d剂量的养精胶囊组方,十一酸睾酮组给予4mg/(kg·d)剂量的十一酸睾酮,西地那非组给予2.5 mg/(kg·d)剂量的西地那非。分别在实验第0、30、60天观察各组大鼠阿扑吗啡诱导的自发勃起反应,记录勃起次数及勃起潜伏期,测定大鼠血清睾酮、黄体生成素水平,并观察大鼠阴茎海绵体组织切片。结果:实验第30天时,所有实验组大鼠阿扑吗啡诱导的勃起次数明显下降,勃起潜伏期延长,有统计学意义(P<0.05)。第60天时,大豆黄酮组(1.39±0.42 vs 2.67±0.33)和大豆黄酮及低剂量养精胶囊组(1.33±0.49 vs 2.83±0.61)大鼠阿扑吗啡诱导的勃起次数明显下降(P<0.05);阿扑吗啡诱导的大鼠勃起潜伏期只有大豆黄酮组[(16.33±3.11)min vs(8.50±0.93)min]和大豆黄酮及低剂量养精胶囊组[(15.50±3.21)min vs(8.63±1.54)min]明显延长(P<0.05),其余各组变化不明显。实验第30天时,所有实验组大鼠血清睾酮、黄体生成素均有显著下降(P<0.05)。实验第60d时,大豆黄酮组[(5.34±0.89)ng/ml vs(1.24±0.30)ng/ml]和大豆黄酮及低剂量养精胶囊组[(5.28±1.12)ng/ml vs(2.07±0.76)ng/ml]血清睾酮变化有统计学意义(P<0.05)。两组的黄体生成素由(3.62±0.37)ng/ml、(3.79±0.28)ng/ml变为(2.09±0.12)ng/ml、(2.17±0.33)ng/ml,显著下降(P<0.05)。切片结果显示对照组大鼠阴茎海绵体内海绵窦数目多,血管清晰可见。大豆黄酮加睾酮组、大豆黄酮加西地那非组和大豆黄酮加中、高剂量养精胶囊组大鼠海绵体与正常对照Objective ： To investigate the effect of Bushengzhuyang Fang （ Yangjing Capsule, YJC） on penile erectile function and its action mechanisms in rats. Methods ： Fifty-six male SD rats were randomly divided into seven groups of equal number： blank control, daidzein, daidzein ＋ testosterone, daidzein ＋ sildenafil, daidzein ＋ low-dose YJC, daidzein ＋ medium-dose YJC, and daidzein ＋ high-dose YJC. The rats in the blank control group were treated intragastrically with normal saline and those in the other groups with daidzein at the dose of 100 mg per kg per day for 30 days. Then the last five groups received additionally testosterone （4 mg per kg per day） , sildenafil （2.5 mg per kg per day）, low-dose YJC, （0. 315 mg per kg per day）, medium-dose YJC （0.63 mg per kg per day）, and high-dose YJC （ 1.26 mg per kg per day）, respectively. At 0, 30 and 60 days of treatment, we observed the apomorphine-induced spontaneous erectile response and pathological changes in the corpus cavernosum of the rats, recorded the number of penile erection and erectile incubation period, and determined the serum levels of testosterone （T） and luteinizing hormone （LH）. Results： At 30 days of treatment, the number of apomorphine-induced erections was decreased, the erectile incubation period prolonged, and the serum levels of T and LH reduced remarkably in all groups of rats （P 〈0. 05 ）. Compared with the findings at 30 days, the number of penile erections was significantly decreased at 60 days in the daidzein group （ 1.39 ± 0.42 vs 2.67±0.33, P 〈 0.05 ） and daidzein ＋ low-dose YJC group （1.33 ±0.49 vs 2.83 ±0.61, P 〈 0.05） ; the erectile incubation period was markedly extended （[16.33±3.11] vs [8.50±0.93] minand [15.50±3.21] vs [8.63±1.54] min, P〈0.05）; and the serum levels ofT （[5.34±0.891 vs [1.24±0.30] ng/ml and [5.28 ＋1.12] vs [2.07 _＋0.76] ng/ml, P〈0.05） and LH （[3.62 ±0.37] vs [ 2.09 ±0.12 ] ng/ml and [ 3.79 ±0.28 ] vs [ 2.17 ± 0.33 ] ng/m

关 键 词：补肾助阳方 植物雌激素 勃起功能下降 

分 类 号：R285.5[医药卫生—中药学]