中药Formula-3抑制RBL-2H3细胞脱颗粒机制的研究  

The inhibitory effects of Herbal Formula-3 on RBL-2H3 cell degranulation and the underlying mechanism

作　　者：李兴永[1] 李建杰[2] 孙宏治[1] 何韶衡[1] 杨平常[1,2] 刘志刚[1,2] 

机构地区：[1]辽宁医学院附属第一医院普外科,锦州121000 [2]深圳大学医学院过敏反应与免疫学研究所,518060

出　　处：《免疫学杂志》2014年第12期1044-1047,共4页Immunological Journal

基　　金：国家自然科学基金(81373176;81271950;31101280);广东省高等学校国际暨港澳台科技合作创新平台项目(2012gjhz0009);深圳市科技计划国际科技合作项目(GJHZ20130408174112021)

摘　　要：目的通过研究中药复方Formula-3(乌梅、灵芝、人参、当归、黄连、干姜)对嗜碱性粒细胞系(RBL-2H3细胞)和SOC通道的作用,探讨Formula-3对肥大细胞脱颗粒的作用机制。方法体外培养大鼠RBL-2H3细胞系制作肥大细胞的模型,通过MTT实验、脱颗粒实验和激光共聚焦(Confocal)实验来研究Formula-3对肥大细胞脱颗粒的作用机制。结果 Formula-3(终质量浓度0.05、0.2、0.8 mg/ml)在急性(0.5 h)和慢性(12 h)作用下呈质量浓度依赖性的抑制致敏RBL-2H3细胞脱颗粒(P<0.01),而对非致敏RBL-2H3细胞没有影响(P>0.05)。Formula-3可显著的抑制SOC通道钙离子内流(P<0.01)。结论 Formula-3通过抑制SOC通道钙离子内流来显著抑制肥大细胞脱颗粒,为治疗过敏性疾病提供理论依据。In order to explore the mechanism of Herbal Formula-3（consists of Fructus pruni mune,Ganoderma Lucidum, Radix ginseng, Corpus radix angelicae sinensis, Rhizoma coptidis and Rhizoma zingiberis officinalis） in inhibiting mast cells degranulation, we cultured RBL-2H3 cell lines in vitro and investigated the effects of Formula-3 on RBL-2H3 cells and SOC channel by MTT and Confocal Methods. We found that Formula-3（final concentration 0.05, 0.2 and 0.8 mg/ml respectively） could inhibit sensitized RBL-2H3 cells degranulation acutely（0.5 h） and chronically（12 h） in a dose-dependant manner（P 〈 0.01）, and Formula-3 could significantly inhibit calcium influx of SOC channel（P 〈 0.01）, but there were no effect on non-sensitized RBL-2H3 cells（P 〉 0.05）. The results suggested that mast cells degranulation can significantly inhibited by Formula-3 through decreasing calcium mobilization of SOC channel, which provided a theoretical basis for the treatment of allergic diseases.

关 键 词：中药Formula-3 肥大细胞 嗜碱性粒细胞系 操纵性钙通道 

分 类 号：R285[医药卫生—中药学]