IL-22诱导小鼠输卵管上皮细胞表达趋化因子的初步研究  

IL-22 induces chemokine expression in murine oviduct epithelial cells

作　　者：许文[1] 李心群[2] 

机构地区：[1]温州医科大学微生物学与免疫学教研室,325035 [2]温州医科大学附属第一医院门诊部,325035

出　　处：《免疫学杂志》2014年第12期1057-1060,共4页Immunological Journal

基　　金：浙江省自然科学基金(Y271067);温州市科技局资助项目(Y20110126)

摘　　要：目的探讨细胞因子IL-22对小鼠输卵管上皮细胞趋化因子CXCL9、CXCL10表达的影响。方法流式细胞术检测体外培养的小鼠输卵管上皮细胞膜表面IL-22R表达水平。重组鼠IL-22(100 ng/ml)刺激小鼠输卵管上皮细胞,ELISA检测不同时间(0、12、24、48、72 h)CXCL9、CXCL10表达情况,Trans AM ELISA检测转录因子STAT3 DNA结合活性。不同质量浓度的重组鼠IL-22(0、25、50、100、200 ng/ml)刺激小鼠输卵管上皮细胞,ELISA检测CXCL9、CXCL10表达情况。结果小鼠输卵管上皮细胞膜表面表达IL-22R。比较6个时间点CXCL9、CXCL10表达差异,发现CXCL9、CXCL10表达具有时间依赖性。比较不同质量浓度IL-22处理下CXCL9、CXCL10表达差异,IL-22 100 ng/ml为最佳干预浓度,且CXCL9、CXCL10表达与IL-22质量浓度具有依赖关系。IL-22刺激组STAT3 DNA结合活性显著升高,与阴性对照组比较有显著差异(P<0.05)。结论 IL-22能显著诱导小鼠输卵管上皮细胞生成并释放CXCL9、CXCL10,且具有时间和剂量依赖性。IL-22 is a key cytokine on mucosal surface, and the role of IL-22 in the genital tract still need more study. This study aimed to investigate the effects of IL-22 on the release of CXCL9 and CXCL10 from murine oviduct epithelial cells（MOECs）. Flow cytometry was used to detect the expression of IL-22 R on MOECs.Then MOECs were stimulated with recombinant mouse IL-22（rm IL-22）, and the supernatants were analyzed by ELISA to quantify the release of CXCL9 and CXCL10 from the cells; the activation of STAT3 in nuclear lysates was measured by Trans AM STAT3 ELISA. The data shown that the membranes of MOECs highly expressed IL-22R; wihle 48 hours is an effective time for CXCL9 and CXCL10 expression after rm IL-22 treatment and the optimal concentration is 200 ng/ml. STAT3 activity was up-regulated in the IL-22 group compared with negative group（P 〈 0.05）. These results indicated that IL-22 can induce CXCL9 and CXCL10 expression in MOECs in a time- and concentration-dependent manner, and the mechanism might relates to the activation of STAT3 signal transduction pathway.

关 键 词：细胞因子 IL-22 小鼠输卵管上皮细胞 趋化因子 

分 类 号：R691.3[医药卫生—泌尿科学]