重组干扰素-α增强口蹄疫重组疫苗诱导滤泡辅助性T细胞的研究  

Recombinant interferon-α enhances the generation of follicular T helper cells induced by foot-and-mouth disease recombinant vaccine

作　　者：苏春霞[1] 段相国[2] 徐广贤[2] 郑洁[1] 

机构地区：[1]宁夏医科大学基础医学院病原生物学与免疫学系,银川750004 [2]宁夏医科大学基础医学院检验学院临床免疫学检验系,银川750004

出　　处：《免疫学杂志》2014年第12期1061-1064,共4页Immunological Journal

基　　金：国家自然科学基金(31001064,81260678,31360600);宁夏自然科学基金(NZ13060);宁夏教育厅高等学校科学研究项目(NGY2012065)

摘　　要：目的通过观察重组干扰素-α(IFN-α)佐剂在口蹄疫(foot and mouth disease,FMD)重组疫苗免疫应答中对滤泡辅助性T细胞(follicular T helper cells,Tfh细胞)的免疫调节作用,为IFN-α佐剂免疫调节的新机制提供实验依据。方法 6周龄雌性Balb/c小鼠分为3组,实验组联合免疫表达猪IFN-α的重组腺病毒(r Ad5po IFN-α)和表达FMDV VP1的重组腺病毒(r Ad5VP1),另设2对照组,分别注射r Ad5VP1和空载体腺病毒(Advirus)。免疫后7 d,应用流式细胞术检测脾脏淋巴细胞中Tfh细胞(CD4+CXCR5+PD-1+)和生发中心(germinal center,GC)B细胞(B220+GL-7+)的增殖分化;real-time PCR技术检测Tfh细胞转录因子Bcl-6m RNA的表达水平;ELISA方法检测血清中细胞因子IL-21蛋白的表达水平。结果与单独免疫r Ad5VP1组相比,联合免疫r Ad5po IFN-α和r Ad5VP1能明显促进FMD重组腺病毒疫苗诱导小鼠Tfh细胞和GC B细胞的增殖分化;重组IFN-α能明显促进FMD重组腺病毒疫苗诱导小鼠Bcl-6 m RNA的表达,提高小鼠血清中IL-21蛋白的分泌水平。结论重组IFN-α能有效增强FMD疫苗诱导小鼠Tfh细胞免疫,为IFN-α佐剂免疫调节作用的新机制提供了理论依据。This study aimed to investigate a novel immune modulatory mechanism of recombinant interferon-α（IFN-α） as an adjuvant in regulating follicular T helper cells（Tfh cells） during foot-and-mouth disease（FMD）recombinant vaccine immune response. Six weeks old female Balb/c mice were divided into 3 groups： the experimental group co-injected with recombinant adenovirus expressing porcine interferon-α（r Ad5 po IFN-α） and FMD recombinant adenovirus expressing FMDV VP1（r Ad5VP1）, while two other control groups were injected with r Ad5VP1 and r Advirus respectively. Seven days after immunization, CXCR5＋PD1＋CD4＋Tfh cell and B220＋GL-7＋germinal center B cells（GC B cells） were examined by flow cytometry; the gene expression level of Bcl-6 m RNA was examined by real-time PCR, and the serum IL-21 secretion level was measured by ELISA. Compared with r Ad5VP1 group, co-injection r Ad5 po IFN-α and r Ad5VP1 group could significantly promote the FMD recombinant vaccine-induced proliferation of Tfh cells and GC B cells, and enhance the expression level of Bcl-6 m RNA and serum level of IL-21. In conclusion, recombinant IFN-α can effectively enhance the FMD vaccine-induced Tfh cells immune response, which providing a experimental basis for immunomodulatory mechanism of recombinant IFN-α as an adjuvant of FMD recombinant vaccine.

关 键 词：滤泡辅助性T细胞 干扰素-Α 口蹄疫疫苗 重组腺病毒 

分 类 号：R392[医药卫生—免疫学]