糖尿病小鼠角膜损害的形态观察  

Morphology observation of corneal injury in diabetic mice

作　　者：杨硕[1] 王娟[1] 万幸[1] 贺恒[1] 李斌[1] 刘磊[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院眼屈光治疗中心,湖北省武汉市430030

出　　处：《眼科新进展》2014年第12期1118-1119,共2页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:81271015);湖北省自然科学基金资助(编号:2009CDB206)~~

摘　　要：目的观察糖尿病小鼠角膜形态损害的特点,为后期进行的糖尿病角膜病变保护因子研究提供研究基础。方法 STZ诱导的糖尿病C57BL/J小鼠,分别于糖尿病成功造模后3个月、6个月时,选取糖尿病小鼠和正常对照组小鼠角膜,石蜡包埋后行HE染色观察形态学变化。结果糖尿病成功造模后3个月、6个月,与正常对照组相比,糖尿病小鼠角膜水肿、基质增厚,角膜溃疡区上皮缺损,溃疡区基质增厚、角膜水肿明显高于非溃疡区。结论糖尿病小鼠造模后3个月即可发生角膜基质病变,6个月时角膜基质改变更加严重。Objective To observe the morphological characteristics of corneal injury in diabetic mice,and provide the research foundation of diabetic keratopathy pro- tective factors for the future study. Methods The diabetic models in C57BL/J mice were induced by bystreptozotocin. The cornea from the diabetic mice and normal control mice were chosen at 3 months and 6 months after model establishment,paraffin was embedded and corneal morphological changes was observed by HE staining. Results Compared with the normal control group, the corneal edema was appeared with thick stroma at 3 months and 6 months after model establishment, the epithelium around the corneal ulcer was incomplete, and the extent of thickening and edema in ulcer area was higher than that in non-ulcer area significantly. Conclusion The corneal stroma has obvious pathological change in diabetic mice at 3 months after model establishment,and which was serious at 6 months.

关 键 词：糖尿病 角膜病变 HE染色 

分 类 号：R587.1[医药卫生—内分泌]