Th17和Treg细胞来源的儿茶酚胺在胶原诱导性关节炎中的作用  

Role of Catecholamines Derived from Th17 and Treg Cells in Type Ⅱ Arthritis Induced by Collagen

作　　者：王小琴[1] 彭聿平[1] 刘展[1] 邱一华[1] 

机构地区：[1]南通大学医学院生理学系,226001

出　　处：《天津医药》2014年第12期1172-1175,I0012,I0013,共6页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(31371182);江苏省高校自然科学研究项目(13KJB310012)

摘　　要：目的探讨胶原诱导性关节炎(CIA)疾病发生发展过程中Th17和Treg细胞来源的儿茶酚胺(CAs)的作用。方法随机将18只雄性DBA/1小鼠分为对照组、CIA模型Ⅰ组(35 d)和CIA模型Ⅱ组(55 d)。Ⅱ型胶原乳剂尾根部注射制备CIA小鼠模型。Real-time PCR法检测淋巴结中Th17细胞的特异性转录因子ROR-γt,相关细胞因子IL-17和IL-22,Treg细胞的特异性转录因子Foxp3,相关细胞因子转化生长因子(TGF)-β以及酪氨酸羟化酶(TH)的m RNA表达。免疫荧光组织化学染色法观察淋巴结中ROR-γt和Foxp3分别与TH、囊泡单胺转运体-2(VMAT-2)和单胺氧化酶(MAO)的共定位情况。结果与对照组相比,CIA模型Ⅰ组和模型Ⅱ组的小鼠淋巴结中ROR-γ、IL-17、TH和IL-22的m RNA表达增加;Foxp3和TGF-β的m RNA表达减少;其中模型Ⅱ组IL-17的m RNA表达与模型Ⅰ组相比有所降低。对照组和CIA模型Ⅰ组及模型Ⅱ组的小鼠淋巴结中存在ROR-γt和Foxp3分别与TH、VMAT-2和MAO的共定位细胞。CIA模型组小鼠淋巴结中ROR-γt/TH、ROR-γt/VMAT-2和ROR-γt/MAO双阳性细胞数增加,模型Ⅱ组中的这些双阳性细胞数与模型Ⅰ组相比有所减少。结论 CIA模型组小鼠淋巴结中Th17细胞合成CAs的能力增强,这种Th17细胞来源的CAs的增加可能在CIA的发生发展过程有一定的抗炎作用。Objective To explore the role of Th17- and Treg-derived catecholamines during collagen-induced ar-thritis（CIA） progression. Methods Eighteen male DBA/1 mice were randomly divided into control group, CIA model groupⅠ（day 35） and CIA model group Ⅱ（day 55）. The CIA models were induced by type Ⅱ collagen（CⅡ） injection from tails.m RNA expression of Th17 specific transcription factor include ROR-γt, cytokines, IL-17 IL-22, Treg specific transcriptionfactor, Foxp3, cytokines, TGF-β and tyrosine hydroxylase（TH） in lymph nodes were examined by real-time PCR. Co-local-ization of ROR-γt or Foxp3, with TH, vesicular monoamine transporter-2（VMAT-2） or monoamine oxidase（MAO） in lymphnodes were observed by immunofluorescence staining. Results In lymph nodes of mice in CIA Ⅰgroup and CIA Ⅱ group,m RNA expression of ROR-γt, IL-17, TH and IL-22 were upregulated, while m RNA expression of Foxp3 and TGF-β ex-pression was downregulated compared to those expression in control group. The upregulated expression of IL-17 was signifi-cantly reduced in CIA Ⅱ group compared with that in CIA Ⅰ group. In the lymph nodes of both intact and CIA mice, co-lo-calization of ROR-γt or Foxp3 with TH, VMAT-2 or MAO was seen in some cells. The numbers of cells that are double-pos-itive of ROR-γt/TH,ROR-γt/VMAT-2 and ROR-γt/MAO IL-17 were increased in CIA groups compared to those in con-trol group. And they are significantly reduced in CIA Ⅱgroup compared with those in CIA Ⅰ group. Conclusion The abili-ty to synthesize catecholamines in Th17 cells was increased in lymph node in mice from CIA groups compared to that in con-trol group. The increased catecholamines production from Th17 cells in lymph nodes may be involved in the anti-inflammato-ry progression in CIA.

关 键 词：关节炎 类风湿 儿茶酚胺类 T淋巴细胞 调节性 酪氨酸单氧化酶 Th17细胞 胶原诱导性关节炎 

分 类 号：R392[医药卫生—免疫学]