miR-155对肝癌细胞增殖的影响  

Effect of miR-155 on Hepatocellular Carcinoma Cell Proliferation

作　　者：南雪[1,2] 曾泉[1,2] 吕洋[1,2] 姚海雷[1,2] 陈琳[1,2] 岳文[1,2] 裴雪涛[1,2] 

机构地区：[1]军事医学科学院野战输血研究所全军干细胞与再生医学重点实验室,北京100850 [2]军事医学科学院华南干细胞与再生医学研究中心,广东广州510005

出　　处：《生物技术通讯》2014年第6期783-786,共4页Letters in Biotechnology

基　　金：国家重点基础研究发展计划(2011CB964804)

摘　　要：目的:在肝癌细胞系中过表达miR-155,研究其对肝癌细胞增殖的影响。方法:将pc DNA3.0-miR-155表达载体瞬时转染Huh7.5.1及Hcclm3肝癌细胞系,通过实时定量PCR技术对miR-155在转录水平的表达进行检测,采用CCK8法及克隆形成实验检测miR-155过表达后对Huh7.5.1及Hcclm3肝癌细胞系增殖的影响。结果:转染细胞后72 h,经实时定量PCR检测,Huh7.5.1及Hcclm3肝癌细胞中成熟miR-155的表达分别上调约431及16倍(P<0.01),说明其能有效高表达;CCK8法及克隆形成实验结果显示,miR-155能够明显促进肝癌细胞增殖(P<0.01)。结论:pc DNA3.0-miR-155转染Huh7.5.1及Hcclm3肝癌细胞系后能高效表达成熟miR-155,同时证明过表达miR-155能使肝癌细胞的增殖受到非常明显的促进。Objective： To study the effect of miR-155 overexpression on hepatocellular carcinoma cell prolifera？tion. Methods： pcDNA3.0-miR-155 was transiently transfected into Huh7.5.1 and Hcclm3 hepatocellular carcino？ma cell lines. RT-PCR assay was used to access the transcription level of miR-155. CCK8 assay and colony-form？ing unit assay were used to evaluate the effect of miR-155 overexpression on Huh7.5.1 and Hcclm3 cell prolifera？tion. Results： miR-155 could be highly efficient expressed 72 h post transfection, and the expression level was accordingly upregulated 431 and 16 folds respectively（P〈0.01）. CCK8 assay and colony-forming unit assay also showed that miR-155 could obviously stimulate hepatocellular carcinoma cell proliferation（P〈0.01）. Conclusion：miR-155 can be highly expressed in Huh7.5.1 and Hcclm3 hepatocellular carcinoma cell lines via pcDNA3.0-miR-155 transfection. Meanwhile, miR-155 overexpression can obviously promote hepatocellular carcinoma cell pro？liferation.

关 键 词：MIR-155 肝癌 细胞增殖 MIR-155 

分 类 号：Q78[生物学—分子生物学] Q25