α-半乳糖苷酶清除猪细胞表面的α-Gal抗原  

Removal of the α-Gal Epitopes of Pig Cell Surface by α-Galactosidase

作　　者：贠志敏 张雪[1] 李素波[1] 檀英霞[1] 冷泠[1] 季守平[1] 高红伟[1] 宫锋[1] 

机构地区：[1]军事医学科学院野战输血研究所血液分子生物学研究室,北京100850

出　　处：《生物技术通讯》2014年第6期849-851,共3页Letters in Biotechnology

摘　　要：目的:探讨脆弱类杆菌来源的基因重组α-半乳糖苷酶清除猪细胞表面α-Gal抗原的作用。方法:用不同浓度的α-半乳糖苷酶酶解猪红细胞、猪胚肾细胞PK15、猪睾丸细胞ST和原代培养的猪成纤维细胞上的α-Gal抗原,酶解温度为26℃,作用时间为2 h;用25μg/m L的FITC-IB4凝集素标记酶解前后的细胞,采用流式细胞仪检测细胞表面α-Gal抗原的清除率。结果:流式细胞检测结果表明,不同组织来源的猪细胞表面的α-Gal抗原的表达量明显不同,所需酶的剂量也不同,但其表面的α-Gal抗原均能被α-半乳糖苷酶清除。结论:脆弱类杆菌来源的α-半乳糖苷酶可以清除猪细胞表面的α-Gal抗原,提示该酶对降低异种移植引起的超急性排斥反应有重要意义。Objective： To investigate effect of recombinant α-galactosidase derived from Bacteroides fragilis re？moving α-Gal epitopes of pig cell surface. Methods： Pig erythrocytes, pig embryonic kidney cells（PK15）, porcine testicular cells（ST） and cells in primary culture of fibroblasts were treated with different dose of α-galactosidase at 26℃ for 2 hours. Treated and non-treated cells were labelled using 25 μg/mL FITC-IB4, and detected by flow cytometry. Results： Our results showed that there were significantly different α-Gal epitopes expression on differ？ent kind of porcine cell surface. So, the different doses of the enzyme were required for cleaning the α-Gal anti？gen on different kinds of cells totally. Conclusion： α-Gal antigen of porcine cell surface could be cleared by α-galactosidase derived from B.fragilis. It is of great significant to reduce hyperacutexenograft rejection.

关 键 词：异种抗原 α-Gal抗原 超急性排斥反应 Α-半乳糖苷酶 

分 类 号：Q503[生物学—生物化学] Q26