番茄环斑病毒纳米荧光颗粒试纸条的研制  

Rapid Detection of Tomato ringspot Nepovirus with Fluorescent Nanoparticles Based Lateral Flow Strip Assay

作　　者：李鑫[1] 刘卉秋[1] 胡强[1] 曹冬梅[1] 曹际娟[1] 

机构地区：[1]辽宁出入境检验检疫局,辽宁大连116001

出　　处：《生物技术通讯》2014年第6期852-854,共3页Letters in Biotechnology

基　　金：辽宁省自然科学基金(201102077);辽宁省博士启动基金(20111149)

摘　　要：目的:研发一种快速、便捷检测番茄环斑病毒(ToRSV)的方法。方法:以荧光纳米颗粒为标记物,采用免疫层析试验方法制备ToRSV荧光纳米颗粒试纸条,在紫外灯下观察试纸条上的荧光信号,作为结果判定依据。结果:用制备的荧光纳米颗粒试纸条检测包括ToRSV在内的9种病毒,仅ToRSV有阳性反应,其余待测样品均呈阴性,表明该试纸条具有较好的特异性;用该荧光试纸条与传统胶体金试纸条进行灵敏度测试时,其灵敏性高于胶体金试纸条1倍以上,且稳定性试验结果良好。结论:ToRSV荧光纳米颗粒试纸条的研制,为快速检测ToRSV提供了有效手段,该方法可用于现场检验,具有广阔的应用前景。Objective： To establish a rapid and simple method for detecting the Tomato ringspot Nepovirus （ToRSV）. Methods： Based on the method of immuno-chromatographic test, fluorescent nanoparticles strip of ToRSV was preparated which using the fluorescent nanoparticles as markers. The fluorescence signal results could be readed under the UV light. Results： The method had good specificity and there were no cross-reaction with non-ToRSV. The sensitivity and stability tests were showed that fluorescent nanoparticles strip higher than doubled colloidal gold test strip. Conclusion： This fluorescent nanoparticles strip assay is a sensitive, convenient, rapid method without the need of special apparatus, so it is of good practical value for the detection of ToRSV in quar？antine or field test site.

关 键 词：番茄环斑病毒 荧光纳米颗粒试纸条 免疫层析 

分 类 号：R392.1[医药卫生—免疫学]