流动注射-光纤化学传感荧光法测定维生素B_2的含量  

Determination of VB_2 tablets using fiber optic chemical sensor fluorescence and flow injection

作　　者：章立华[1] 艾尼娃尔.艾克木 艾尔肯.依不拉音 

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830011 [2]新疆医科大学中心实验室,新疆乌鲁木齐830011

出　　处：《应用化工》2014年第11期2104-2106,共3页Applied Chemical Industry

基　　金：国家自然科学基金项目(21162030)

摘　　要：结合流动注射，流通池和光纤化学传感技术，建立在线荧光法检测系统。将维生素B2片溶解于1%冰醋酸水溶液中，流动注射法把维生素B2溶液注入检测系统，在流通池中进行荧光检测维生素B2溶液的含量。结果表明，维生素B2最大发射波长为530.93 nm，质量浓度在2.6×10-3~13.2×10-3 mg/mL与荧光强度呈良好线性关系，检测线为4.74×10-5 mg/mL，定量线为9.07×10-4 mg/mL，日内精密度为1.67%，日间精密度为2.23%，回收率为98.04%~102.62%。Set up a fluorescence detection system combined with flow injection and fiber optic chemical sensor technology. The VB2 tablets dissolved in 1% acetic acid solution,which is injected into the detec-tion system. The fluorescence is detected in the flow cell,thereby the content of VB2 solution is deter-mined. Results showed that the maximum emission wavelength is 530. 93 nm. There is a good linear rela-tionship between the fluorescence intensity and concentration ranged from 2 . 6 × 10 -3 to 13 . 2 × 10 -3 mg/mL,the test line is 4. 74 × 10 -5 mg/mL,the quantitative line is 9. 07 × 10 -4 mg/mL,intra-day precision is 1 . 67%,during the day precision is 2 . 23%,the recovery is 98 . 04% ~102 . 62%.

关 键 词：光纤化学传感 流动注射 VB2 荧光法 

分 类 号：TQ016.5[化学工程]