反硝化聚磷菌菌剂种子液制备条件及除磷机理  

Preparation of Denitrifying Phosphorus Accumulating Bacterial Seed Liquid and Analysis of Phosphorus Removal Mechanism

作　　者：张文艺[1] 陈晶[1] 邓文[1] 陈萍[1] 周新程 

机构地区：[1]常州大学环境与安全工程学院,江苏常州213164

出　　处：《土木建筑与环境工程》2014年第6期99-105,共7页Journal of Civil,Architectural & Environment Engineering

基　　金：江苏省自然科学基金(BE2012640);"十二五"国家水体污染控制与治理科技重大专项(2012zx07301-001)

摘　　要：为获取高效反硝化聚磷菌（DNPAOs）菌剂种子液以控制水体富营养化，从安徽省天长市污水处理厂氧化沟活性污泥中分离得到1株具有高效脱氮除磷功能的恶臭假单胞菌B8（ Pseudo‐monas putida sp ．）。利用多聚磷酸盐颗粒（Poly‐P）染色得到具有高Poly‐P含量的B8菌剂种子液。其适宜的培养条件为：p H 6.5～7.0，温度30～32℃，溶解氧相当于70％～88％饱和溶解氧（摇床转速120～140 r／min），培养时间15～20 h。所得的反硝化聚磷菌种子液B8具有良好的同步反硝化除磷效果，对于污水厌氧／缺氧（ A／A ）处理，其聚磷率、硝酸盐氮去除率分别达到89.73％和53.48％，而经厌氧／好氧（A／O）处理磷去除率最高可达94.09％。通过B8胞外聚合物（EPS）提取与磷去除实验表明其对磷酸盐去除源于B8胞内的吸收，而非胞外的生物吸附。In order to control the water eutrophication , microorganism agents with high capability of denitrifying phosphorus accumulation are required .A denitrifying poly‐phosphate accumulating organisms （DNPAOS ） ,B8（Pseudomonas putida sp .）was isolated from activated sludge in oxidation ditch of Anhui Tianchang wastewater treatment plant .Intracellular polyphosphate granule （Poly‐P）dyeing method was used to optimize the culture conditions of Poly‐P‐producing Pseudomonas putida strain B8 seed liquid .T he results showed that the appropriate culture conditions were as follow ：pH value and temperature ranged from 6.5 to 7.0 and 30~32 ℃m respectively .The saturation values of dissolved oxygen varied from 70%to 88% （when velocity was in range of 120~140 r/min） ,15~20 h proved to be the cultivation time .The seed liquid produced by B8 showed high phosphorus uptake rate in the processes of anaerobic‐anoxic and anaerobic‐aerobic in wastewater （89.73% and 94.09% respectively）In the anaerobic‐anoxic process ,the highest nitrate removal efficiency was 53.48% .Extracellular polymeric substances（EPS）extracting method and analysis of phosphorus removal characterization revealed that phosphorus removal was mainly caused by intracellular uptake ,rather than by extracellular biological adsorption .

关 键 词：反硝化聚磷菌 菌剂 种子液 多聚磷酸盐颗粒 污水处理 

分 类 号：X703.1[环境科学与工程—环境工程]