复方苦豆子灌注液总生物碱含量测定方法的建立  

Establishment of Method for Determination of Total Alkaloids in Compound Sophora alopecuroides Perfusion

作　　者：辛任升 刘宇[2] 张超[2] 梁剑平[1,2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室,甘肃省新兽药工程重点实验室,甘肃兰州730050

出　　处：《动物医学进展》2014年第12期89-92,共4页Progress In Veterinary Medicine

基　　金：“十二五”农村领域国家科技计划项目(2011AA10A214)

摘　　要：为建立复方苦豆子灌注液总生物碱含量的测定方法,采用酸性染料比色法,以氧化苦参碱为对照品进行含量测定,检测波长420nm的吸光度。结果显示,氧化苦参碱在2.0μg/mL^10μg/mL范围内与吸光度呈良好线性关系,回归方程为A=0.005 8C+0.111 6,r=0.999 2,氧化苦参碱的平均回收率为99.6%,RSD=2.20%(n=5)。该方法简便灵敏、准确可靠,可作为复方苦豆子灌注液的质量控制方法。To establish a method for the determination of total alkaloids in Compound Sophora Alopecui- oides Perfusate,oxymatrine as the reference, the total alkaloids were determined by acid dye colorimetry at 420 nm. The results showed that the linear ranges of the oxymatrine were 2.0μg/mL-10μg/mL. The re- gression equation was A=0. 005 8C＋0. 111 6, （r=0. 999 2）. The average recovery rate was 99.6% （RSD =2.20%）. The method was simple, accurate and reproducible.

关 键 词：复方苦豆子灌注液 酸性染料比色法 总生物碱 氧化苦参碱 

分 类 号：S853.72[农业科学—临床兽医学]