宁夏地区奶牛乳房炎源性放射根瘤菌的分离鉴定与药敏试验  

Isolation,Identification and Drug Sensitivity Test of Rhizobium radiobacter from Cows with Mastitis in Ningxia Area

作　　者：康晓冬[1] 刘溪源[2] 杨慧君[2] 王建东[1] 

机构地区：[1]宁夏农林科学院草畜工程技术研究中心,宁夏银川750002 [2]宁夏大学农学院,宁夏银川750021

出　　处：《动物医学进展》2014年第12期92-95,共4页Progress In Veterinary Medicine

基　　金：宁夏科技支撑计划项目:宁夏地区奶牛乳房炎主要致病菌分子病原学快速诊断技术研究

摘　　要：从宁夏地区部分奶牛场采集164份患有乳房炎奶牛的乳样,采用VITEK-2全自动微生物鉴定系统进行病原菌的分离鉴定,共分离鉴定出放射根瘤菌30株,分离率高于奶牛乳房炎常见的病原菌溶血葡萄球菌、巴氏杆菌、金黄色葡萄球菌等;15种抗菌药物的药敏试验结果表明,放射根瘤菌对阿莫西林、林可霉素、先锋霉素Ⅴ、头孢曲松已产生不同程度的耐药性,而其他磺胺类、氨基糖苷类、喹诺酮类、头孢类中的11种药物则表现出较好的抑菌效果。164 milk samples were collected from cow mastitis cases which broke out in Ningxia area.Then i-solated pathogenic bacteria were identified by VITEK-2 automatic microbial identification system.The de-tection results showed that 30 strains of Rhizobium radiobacter were isolated and identified,and the isola-tion rate was higher than that of usual pathogenic bacteria which led to cow mastitis,such as Staphylococ-cus haemolyticus ,Pasteurella and Staphylococcus aureus .The results of drug sensitivity test to 15 kinds of antibacterial drugs showed that the isolated Rhizobium radiobacter already had different resistance de-gree to amoxicillin,lincomycin,cephalosporin V and ceftriaxone.Meanwhile,we also found that other 11 drugs which belong to sulfanilamide groups,aminoglycosides,quinolones and cephalosporins displayed good antibacterial effects.

关 键 词：放射根瘤菌 分离鉴定 药敏性分析 

分 类 号：S857.26[农业科学—临床兽医学]