罗汉果果实总RNA提取方法的比较研究  

Comparison of methods for total RNA extraction from Siraitia grosvenorii fruits

作　　者：韦荣昌[1,2] 马小军[2] 白隆华[1] 吴庆华[1] 施力军[1] 潘丽梅[1] 冯世鑫[1] 莫长明[1] 

机构地区：[1]广西药用植物园,广西南宁530023 [2]中国医学科学院&北京协和医学院,药用植物研究所,北京100193

出　　处：《浙江农业学报》2014年第6期1629-1634,共6页Acta Agriculturae Zhejiangensis

基　　金：国家十二五科技支撑计划项目(2011BAI01B03);国家自然科学基金项目(30960500;81373914);广西自然科学基金项目(2013GXNSFDA019021)

摘　　要：针对罗汉果果实富含多糖和多酚类物质的特点,以广西罗汉果代表种质青皮果种的果实为材料,比较了改良Trizol法、改良异硫氰酸胍法、改良CTAB法和常规Trizol法4种不同的总RNA提取方法的提取效果。结果表明,改良Trizol法所提取的RNA呈现28S rRNA、18S rRNA和5S rRNA 3条清晰的谱带,且28S条带宽度接近18S的2倍,纯度高(D260/D280=2.01;D260/D230=2.02),完整性好(RIN=9.50),得率为(260.00±19.47)μg·g-1。RT-PCR结果进一步表明,改良Trizol法提取的RNA完全能够用于后续的分子生物学研究。Total RNA was isolated from Siraitia grosvenorii fruits of Qingpiguo in Guangxi by the methods of Trizol,improved Trizol,modified guanidine thiocyanate and CTAB,with rich polyphenols and polysaccharide. The results showed that RNA isolated by the improved Trizol showed three clear bands of 28 S rRNA,18 S rRNA and 5S rRNA,respectively,and the 28 S was about twice in length as 18 S,which indicated that little RNA was decomposed and the purity of RNA was very high（ D260/ D280= 2. 01; D260/ D230= 2. 02）. In addition,the RNA integrity（ RIN） and yield rate of RNA extracted by the improved Trizol was 9. 50 and（ 260. 00 ± 19. 47） μg·g^-1,respectively. RT-PCR amplification also showed that the quality of RNA isolated from the improved Trizol can meet the demands of molecular biology experiment.

关 键 词：罗汉果果实 总RNA提取 改良Trizol法 

分 类 号：R931[医药卫生—生药学]