三种不同涂片法对分枝杆菌涂片阳性率的影响  

INFLUENCES OF THREE SMEAR METHODS ON THE POSITIVE RATE OF MYCOBACTERIA

作　　者：刘燕文[1] 曾少芳[1] 吴龙章[1] 

机构地区：[1]广州市胸科医院,广东广州510095

出　　处：《现代医院》2014年第11期88-89,共2页Modern Hospitals

摘　　要：目的探讨液基夹层杯法、荧光染色法和抗酸染色法检测痰液分枝杆菌的应用价值。方法应用液基夹层杯法、荧光染色法和抗酸染色法分别检测311例临床痰液标本,统计分析3种不同方法的分枝杆菌阳性检出率。结果夹层杯法、荧光染色法和抗酸染色法的阳性检出率分别为15.8%、20.9%、11.9%,其中荧光染色法阳性检出率最高,且操作简单快速,夹层杯法次之,抗酸染色法阳性检出率最低。结论总体来说,荧光染色法操作简单、快速、阳性率较高,是直接检测痰液分枝杆菌比较理想的方法。Objective To evaluate the influences of three smear methods of liquid- based sandwich cup method,fluorescence staining and acid- fast staining on detection of mycobacteria in sputum. Methods 311 cases of clinical sputum specimens were detected by the three different methods,and their detection rates of mycobacteria positive were statistically analyzed. Results The positive detection rates by liquid- based sandwich cup method,fluorescence staining and acid- fast staining were 15. 8%,20. % and 11. 9%,respectively. The positive rate of fluorescence staining with simple and rapid operation was the highest,and that of acid- fast staining was the lowest.Conclusion Among the three methods,fluorescence staining is a more appropriate method for the detection of mycobacteria in sputum for its higher positive rate,rapid and simple operation.

关 键 词：夹层杯法 金胺O荧光染色 抗酸染色 分枝杆菌 

分 类 号：R378.91[医药卫生—病原生物学]