大麦类病斑突变体bspl1的遗传及生理生化分析  

Genetic and Physiological Analyses of Barley Lesion Mimic Mutant bspl1

作　　者：孙扬[1] 陆文怡[1] 张艳[1] 王广洋 孙佳丽[1] 薛大伟[1] 张晓勤[1] 

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310036

出　　处：《杭州师范大学学报（自然科学版）》2014年第6期602-605,611,共5页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：浙江省自然科学基金项目(Y14C130029);国家级大学生创新创业训练计划项目(201310346005)

摘　　要：对甲基磺酸乙酯(EMS)诱变大麦品种ZJU3获得的一个类病斑突变体(bspl 1)进行了遗传和生理分析.遗传分析结果显示,该表型由隐性单基因控制;苗期叶片生理生化分析表明,bspl 1中SOD、POD以及CAT的活性较野生型ZJU3均明显升高.通过比较bspl 1和野生型ZJU3在生理生化水平上的差异,推测类病斑的产生与叶片中活性氧的堆积相关.Barley lesion mimic mutant bspl 1,which is derived from Ethylmethylsulfone mutagenesis of ZJU3,is made genetic and physiological analysis.The genetic analysis indicates that the phenotype of bspl 1 is controlled by a recessive monogene.The physiological and biochemical analyses of seedling leaves show that the activity of SOD,POD and CAT in bspl 1is significantly higher than that in wild type ZJU3.The results demonstrate that the lesion appearance of bspl 1 is related with the accumulation of reactive oxygen species in leaves.

关 键 词：大麦 类病斑突变体 活性氧 遗传分析 

分 类 号：Q94[生物学—植物学] S512.3[农业科学—作物学]