国产六种小型蝰科毒蛇蛇毒免疫原性比较  

Comparison of the Immunogenicity of Venoms from Six Small Domestic Species of Viperidae Venomous Snakes

作　　者：张艳霞[1] 刘虞[1] 蔡梦思 单琳琳[1] 高建芳[1] 

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310036

出　　处：《杭州师范大学学报（自然科学版）》2014年第6期606-611,共6页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：浙江省自然科学基金项目(Y14C030007);杭州师范大学实验室开放项目(SYSKF2013031);杭州师范大学研究生创新基金项目

摘　　要：以国产小型蝰科毒蛇短尾蝮(Gloydius brevicaudus)、岩栖蝮(Gloydius saxatilis)、乌苏里蝮(Gloydius ussuriensis)、山烙铁头(Ovophis monticola)、原矛头蝮(Protobothrops mucrosquamatus)和竹叶青(Trimeresurus stejnegeri)蛇毒为对象,用国产商用抗蝮蛇毒血清和抗五步蛇毒血清分别检测这6种蛇毒的免疫原性.间接ELISA和western blot两种方法的检测结果表明:6种蛇毒有共同的抗原组分,且在免疫反应中大部分组分表现出免疫原性;同种蛇毒和抗血清之间显示出最高的免疫结合强度;近缘物种间的免疫结合强度更接近;随着抗体浓度的升高,免疫结合强度增加.抗蝮蛇毒血清对短尾蝮、乌苏里蝮和岩栖蝮蛇毒的免疫中和效价高于抗五步蛇毒血清;两种抗蛇毒血清中和山烙铁头、原矛头蝮和竹叶青蛇毒的效价相近.The immunogenicity of venoms from six small domestic species of Viperidae venomous snakes（Gloydius brevicaudus, Gloydius saxatilis, Gloydius ussuriensis, Ovophis monticola, Protobothrops mucrosquamatus and Trimeresurus stejnegeri）is investigated in this study.The immunological cross-reactions between venoms and two commercial antivenoms（G.brevicaudus antivenom and D.acutus antivenom）are evaluated by ELISA and western blot.The results show that venoms from these six snakes are shared many common antigenic components,and most venom components express antigenicity. Homologous venom and antivenom express the highest immunoreactivity. The immunoreactivity of closely related species is more similar.Along with the rising of antivenom concentration,the immunoreactivity increases.The G.brevicaudus antivenom is more effective in neutralizing the venoms of G.brevicaudus,G.saxatilis and G.ussuriensis than D.acutus antivenom.Neutralization titer of these two commercial antivenoms against O.monticola,P.mucrosquamatus and T.stejnegeri is similar.

关 键 词：蛇毒 抗蛇毒血清 免疫原性 蝰科 

分 类 号：Q956[生物学—动物学]