多壁碳纳米管长期作用对活性污泥系统的影响  

Long-Term Effects of Multi-Walled Carbon Nanotubes on Activated Sludge System

作　　者：王玉琳[1] 闻韵[2] 王晓慧[1] 杜志泽[1] 海热提[1] 李媛[1] 

机构地区：[1]北京化工大学、北京市水处理环保材料工程技术中心,北京100029 [2]洛阳理工学院土木工程系,河南洛阳471023

出　　处：《环境科学研究》2014年第12期1486-1492,共7页Research of Environmental Sciences

基　　金：北京市科技计划项目(Z121100001512008);环境模拟与污染控制国家重点联合实验室专项(13K06ESPCT)

摘　　要：为研究MWCNTs(multi-walled carbon nanotubes,多壁碳纳米管)对活性污泥长期作用的影响,建立3组(每组包括3个平行反应器)序批式活性污泥反应器,研究了2种质量浓度(1、20 mg/L)的MWCNTs长期(120 d)作用对活性污泥系统废水处理效果的影响,并采用Illumina MiSeq高通量测序技术研究MWCNTs长期作用后活性污泥中细菌群落结构的变化.结果表明:20 mg/L的MWCNTs对生物脱氮效果存在明显的抑制作用,TN平均去除率由83.4%降至71.7%;1 mg/L的MWCNTs长期作用后TN平均去除率由83.7%降至79.7%.1和20 mg/L的MWCNTs对磷的去除均有抑制作用,TP平均去除率分别由98.0%和98.2%降至52.0%和34.0%.Illumina MiSeq高通量测序结果表明,不同质量浓度的MWCNTs长期作用后降低了活性污泥中细菌群落的多样性.同时,活性污泥中细菌群落结构也发生了变化,其中α-Proteobacteria和γ-Proteobacteria的丰度升高,Bacteroidetes的丰度降低.1 mg/L的MWCNTs长期作用可致以Defluviicoccus和Micropruina为代表的一类聚糖菌(glycogen-accumulating organisms,GAOs)丰度显著升高,20 mg/L的MWCNTs长期作用可致16个核心属的丰度明显降低.Three groups of sequencing batch reactors （SBRs） were operated to investigate the long-terra effects of multi-walled carbon nanotubes （MWCNTs） on activated sludge systems. Each group was set up in triplicate. The long-term effects of 1 and 20 m/L MWCNTs on nutrient removal of activated sludge process were investigated. In addition, bacterial community structure in activated sludge was studied using MiSeq high-throughput sequencing. It was found that 20 mg/L MWCNTs adversely affected the performance of biological nitrogen removal, which significantly reduced the average TN removal efficiencies from 83.4% to 71.7% , and average TN removal efficiencies from 83. 7% to 79. 7% , after long-term exposure to 1 mg/L MWCNTs. In addition, both I and 20 mg/L MWCNTs inhibited biological phosphorus removal, with the average TP removal efficiencies decreasing from 98.0% to 52.0% and from 98.2% to 34. 0% , respectively. MiSeq sequencing indicated that 1 and 20 mg/L MWCNTs obviously decreased the diversity of bacterial community in activated sludge. MWCNTs differentially enhanced the abundance of ct-Proteobacteria and 3,-Proteobacteria, and reduced the abundance of Bacteroidetes. The long-term effects of 1 mg/L MWCNTs significantly enhanced the abundance of glycogen accumulating organisms （GAOs） （i. e. Defluviicoccus and Micropruina）, which are thought to out-compete the polyphosphate-aecumulating organisms （PAOs） in activated sludge communities removing phosphate. The long-term effects of 20 mg/L MWCNTs significantly decreased the abundance of 16 core genera.

关 键 词：多壁碳纳米管 活性污泥 长期作用 细菌群落结构 

分 类 号：X592[环境科学与工程—环境工程]