响应面优化淫羊藿抗氧化多酚超声提取工艺  

Optimization of Ultrasonic-assisted Extraction of Antioxidant Polyphenols from Epimedii Folium by Response Surface Methodology

作　　者：毛白杨 郑洁[2] 赵晓红 何陈丹 徐秀泉[3] 

机构地区：[1]亚邦医药研究所有限公司,江苏常州213163 [2]江苏大学京江学院,江苏镇江212013 [3]江苏大学药学院,江苏镇江212013

出　　处：《广州化工》2014年第23期64-66,共3页GuangZhou Chemical Industry

基　　金：江苏省中医药管理局项目(LZ13244);江苏大学大学生科研立项项目(12C210)

摘　　要：以总多酚得率和DPPH自由基清除率为指标,采用响应面设计法优化淫羊藿抗氧化活性多酚超声提取工艺。结果表明以多酚得率为指标时,最佳工艺条件为:57%乙醇,料液比28 m L/g,在50℃下超声提取25 min,总多酚得率为34.58 mg/g;以DPPH自由基清除率为指标时,最佳工艺条件为:58%乙醇,料液比30 m L/g,在50℃下超声提取22 min,DPPH自由基清除率为88.4%。The ultrasonic-assisted extraction processes of antioxidant polyphenols from Epimedii Folium was optimized by response surface methodology with the extraction rate of polyphenols and scavenging activity of DPPH as response value.The result showed that the optimal extraction conditions were ethanol concentration 57%, liquid-material ratio of 28 mL/g, extraction temperature 50℃, and extraction time 25 min for total polyphenols （34.58 mg/g） , ethanol concentration 58%, liquid-material ratio of 30 mL/g, extraction temperature 50 ℃, and extraction time 22 min for scavenging activity on DPPH free radical（88.4%）.

关 键 词：淫羊藿 抗氧化活性多酚 超声提取 响应面 

分 类 号：R284.2[医药卫生—中药学]