梅毒螺旋体重组蛋白Tp0965的表达与鉴定  

Expression and Identification of Recombinant Protein Tp0965 of Treponema Pallidum

作　　者：周秀萍 杨长顺[2] 牛淑会[3] 李树平 阳大庆 付祥 曾铁兵[3] 

机构地区：[1]湖南医药学院,湖南怀化418000 [2]湖南省怀化市第一人民医院 [3]南华大学医学院

出　　处：《中国医学创新》2014年第33期15-17,共3页Medical Innovation of China

基　　金：国家自然科学基金项目(81273322);湖南省教育厅基金项目(11C1014)

摘　　要：目的:研究梅毒螺旋体(Tp)重组蛋白Tp0965(r Tp0965)的表达并鉴定其免疫反应性,为进一步探讨其在梅毒血清学诊断与疫苗中的应用价值奠定基础。方法:构建Tp0965原核表达重组体p ET-28a(+)/Tp0965,诱导转化宿主菌表达重组蛋白,Ni-NTA亲和层析法纯化蛋白,Western blot鉴定其免疫反应性。结果:成功构建了原核表达重组体p ET-28a(+)/Tp0965,经诱导高效表达了一分子量约为43 KDa的可溶性重组蛋白,纯化蛋白纯度>95%;Western blot显示该纯化蛋白能被梅毒患者血清特异性识别。结论:高效表达了可溶性r Tp0965,该重组抗原有良好的免疫反应性。Objective：To express and identify immunoreactivity of recombinant Tp0965（r Tp0965） protein of Treponema pallidum（Tp）, provid a basis for further investigation of its significance in syphilis serodiagnosis and vaccine.Method：The prokaryotic expression recombinant p ET-28a（＋）/Tp0965 was constructed and induced to express recombinant proteins which then were purified with Ni-NTA affinity chromatography. Western blot was used to identify immunoreactivity of r Tp0965.Result：The recombinant p ET-28a（＋）/Tp0965 was constructed successfully and a soluble recombinant protein with approximate 43 KDa molecular weight was expressed efficiently in E.coli with more than 95% of purity. Western blot indicated that purified proteins were able to be recognized specially by pooled sera from syphilis patients.Conclusion：The recombinant protein Tp0965 is expressed efficiently in E.coli and has good immunoreactivity.

关 键 词：梅毒 梅毒螺旋体 Tp0965 重组抗原 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]