分子信标技术在 ABO 血型基因型检测方面的重要应用  

The important application of molecular beacon technology in the ABO blood group genotyping

作　　者：张硕[1] 孙海燕[1] 王诗铭[1] 宋晓[1] 陈红岩[1] 卢大儒[1] 

机构地区：[1]复旦大学生命科学学院遗传工程国家重点实验室,上海200433

出　　处：《国际遗传学杂志》2014年第6期282-285,295,共5页International Journal of Genetics

摘　　要：目的：人类 ABO 血型的检测在医学和法学领域都有着非常重要的意义，该研究目的在于发展一种利用非血生物学样本进行人体 ABO 血型的精确检测方法。方法该项研究中，选取漱口水、毛发和指甲3种生物样本，采用饱和氯化钠法进行基因组 DNA 的抽提，设计特异性引物和荧光标记的探针，通过分子信标检测技术法进行血型的精确检测。结果漱口水、毛发和指甲3种样本中均能成功抽提得到基因组 DNA ，分子信标技术利用基因组DNA能够快速完成 ABO 血型精确分型，分型结果与传统的免疫学方法一致，且能准确区分血型的纯合型和杂合型。结论利用漱口水、毛发和指甲等非血生物学样本，分子信标技术能精确完成个体 ABO 血型的检测，简便快捷，灵敏度高。Objective The detection of ABO blood group is quite important in medical and foren-sic sciences .Our study was aimed to establish an accurate detection of ABO blood group by testing human biological samples other than blood .Methods In this study , we collected three kinds of human biologi-cal samples , i .e ., mouthwash , hair , and nails , extracted DNA by using saturated sodium chloride method , and detected blood group genotype by molecular beacon .Results By this protocol , we get ge-nomic DNA from all the three sample kinds successfully .Molecular beacon can genotype the ABO gene and distinguish the homozygous and heterozygous accurately .The result is consistent with the results of the conventional immunological method .Conclusion Our research demonstrated that ABO blood group can be detected accurately through the DNA of non-blood biological samples by molecular beacon , which is a rap-id and reliable genotyping approach .

关 键 词：非血样本 基因组DNA ABO血型 血型检测 分子信标技术 

分 类 号：R440[医药卫生—诊断学]