支原体糖基转移酶对哺乳动物细胞的影响  

The effects of mycoplasmal glycosyltransferase on mammalian cells

作　　者：刘文斌[1] 李睿[2] 曾驰[2] 程清洲[1] 

机构地区：[1]武汉轻工大学医学技术与护理学院,湖北武汉430023 [2]武汉轻工大学生物与制药工程学院,湖北武汉430023

出　　处：《武汉轻工大学学报》2014年第4期27-31,共5页Journal of Wuhan Polytechnic University

摘　　要：支原体是引起人类多种疾病的微生物,常常寄居于人体细胞、组织中。通过细胞生长曲线、粘附实验、抗饥饿实验、抗氧化实验及Western blot方法,初步探讨了发酵支原体糖基转移酶对哺乳动物细胞的影响。结果表明,支原体糖基转移酶对细胞生长没有太大的影响,但是抑制了细胞粘附及抗氧化能力,促进了细胞的抗饥饿能力。将有助于让人们更深入地了解支原体如何引起人类及其它哺乳动物多种疾病的细胞和分子机制。Mycoplasmas are those microorganisms that inhabit human cells and tissues, and cause some kinds of dis- eases. This study investigated the effects of Mycoplasma fermentans glycosyhransferase on mammalian cell lines by tests of cell proliferation, adhesion, anti-starvation, anti-oxidization and the assays of Western blot. The results in- dicate that mycoplasmal glycosyltransferase has no apparent effect on cell proliferation, but inhibits cell adhesion and anti-oxidization ability, and promotes the cellular ability of anti-starvation. This study is helpful for people to understand deeply the cellular and molecular mechanisms of diseases caused by mycoplasmas.

关 键 词：发酵支原体 糖基转移酶 细胞增殖 细胞粘附 抗饥饿 

分 类 号：Q2[生物学—细胞生物学]