富氢盐水对重度烫伤延迟复苏大鼠肝脏的作用  

Effects of hydrogen-rich saline on liver of severely scalded rats with delayed resuscitation

作　　者：刘德贵[1] 王晓娟[1] 杨勇[1] 蒋金恒[1] 李先慧[1] 薛刚[1] 

机构地区：[1]成都军区总医院烧伤整形科,成都610083

出　　处：《中华烧伤杂志》2014年第6期506-511,共6页Chinese Journal of Burns

基　　金：国家自然科学基金（8J101427）

摘　　要：目的 探讨富氢盐水对重度烫伤延迟复苏大鼠肝脏的影响. 方法 取24只SD大鼠,应用台式控温烫伤仪制成40％ TBSAⅢ度烫伤模型,按随机数字表法分为富氢盐水组、乳酸钠林格液组,每组12只.伤后6h开始,2组大鼠分别采用富氢盐水（自制,浓度为0.6 mmol/L）、乳酸钠林格液,均按4 mL＆#183; kg-1＆#183;％TBSA-1进行腹腔注射补液,持续到伤后48 h,伤后第2个24 h补液量为伤后第1个24 h的一半.伤后6（补液前）、12、24、48 h,每组分别取3只大鼠行心脏采血,处死后取肝组织.HE染色观察肝组织病理学变化,细胞计数板行肝组织中性粒细胞计数,全自动生化分析仪检测血清AST、ALT水平,ELISA法检测肝组织中TNF-α、IL-1β、IL-6以及8羟基脱氧鸟苷（8-OHdG）的含量,分光光度计检测肝组织中丙二醛的吸光度值并定量.对数据行重复测量方差分析和LSD-t检验. 结果 伤后48 h,与乳酸钠林格液组比较,富氢盐水组大鼠肝组织炎性细胞浸润减少,肝细胞充血减轻.伤后12、24、48 h,富氢盐水组大鼠肝组织中性粒细胞数分别为（25.3±1.8） ×10^5、（19.6±0.6） ×10^5、（14.1±3.2）×10^5个/mL,显著低于乳酸钠林格液组的（31.9±2.0）×10^5、（30.9±2.2） ×10^5、（23.8±3.0） ×10^5个/mL（t值分别为5.6、7.6、8.7,P值均小于0.05）.伤后6、12h,2组大鼠血清AST、ALT水平以及肝组织TNF-α、IL-1 β、IL-6含量均相近（t值分别0.3 ～3.9与0.9 ～3.8,P值均大于0.05）.伤后24、48 h,富氢盐水组大鼠血清AST与ALT水平分别为（308 ±24）、（210±15） U/L与（93±7）、（70 ±5） U/L,显著低于乳酸钠林格液组的（541 ±39）、（505±18）U/L与（156 ±9）、（166 ±21） U/L（t值分别为17.5、16.7与30.3、6.9,P值均小于0.05）.伤后24、48 h,富氢盐水组大鼠肝组织TNF-α、1L-1 β、IL-6含量分别为（20.7±1.6）、（13.7±1.5）pg/mg,（7.7±1.5）、（6.3±1.2）pg/mg与（8.7±1.2）、（6.0±2.0）pg/mg,�Objective To explore the effects of hydrogen-rich saline （HS） on liver of severely scalded rats with delayed resuscitation.Methods Twenty-four SD rats were inflicted with 40% TBSA fullthickness scald using a temperature-controlled scalding apparatus.The injured rats were divided into lactated Ringer＆#39;s solution （RS） and HS groups according to the random number table,with 12 rats in each group.Rats in groups RS and HS were respectively resuscitated with an intraperitoneal injection of 4 mL ＆#183; kg-1 ＆#183; % TBSA-1 of RS or HS （self-prepared,with concentration of hydrogen 0.6 mmol/L） 6 hours after injury up to 48 hours after scald.The infusion volume of the second 24 hours after injury was a half of that of the first 24 hours.At post scald hour （PSH） 6 （before resuscitation）,12,24,and 48,blood was collected from the heart of 3 rats in each group,and then the rats were sacrificed for harvesting liver tissue.The pathological change in liver tissue was observed with HE staining.The number of hepatic neutrophils was counted with a hematocytometer.Serum levels of AST and ALT were determined with full-automatic biochemical analyzer.Contents of TNF-α,IL-1β,IL-6,and 8-hydroxy-2＇ -deoxyguanosine （8-OHdG） in liver tissue were determined with ELISA.Absorbance value of malondialdehyde （MDA） in liver tissue was detected and quantified with spectrophotometer.Data were processed with analysis of variance of repeated measurement and LSD-t test.Results At PSH 48,moderate infiltration of inflammatory cells and hepatic hyperemia were observed in rats of group HS as compared with group RS.At PSH 12,24,and 48,the number of neutrophils in group HS was respectively （25.3 ± 1.8） ×10^5,（19.6 ± 0.6） ×10^5,and （14.1 ± 3.2） ×10^5 cells per mililitre,and they were significantly lower than those in group RS [（31.9 ± 2.0） ×10^5,（30.9 ± 2.2） ×10^5,and （23.8 ±3.0） ×10^5cells per mililitre,with t values respectively 5.6,7.6,and 8.7,P values below 0.05].At PSH 6 and 12,t

关 键 词：烧伤 肝 氢 延迟复苏 

分 类 号：R644[医药卫生—外科学]