动物活体成像生物发光间充质干细胞系的构建  

Establishment of mesenchymal stem cell lines with optical in vivo imaging ability and bioluminescence

作　　者：陈洁玉[1] 施小凤[1] 

机构地区：[1]江苏大学附属医院血液科,镇江212001

出　　处：《上海交通大学学报（医学版）》2014年第11期1705-1709,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：镇江市科技计划项目(SH2012029);中国博士后科学基金项目(2013M541528)~~

摘　　要：目的建立稳定表达荧光素酶的小鼠骨髓间充质干细胞(MSCs)系。方法用携带荧光素酶基因的慢病毒GV260感染F3代MSCs系OP9细胞,通过嘌呤霉素筛选建立稳定表达荧光素酶的OP9 luc+细胞;检测荧光素酶报告基因的活性,观察其表达效果;将OP9 luc+细胞经尾静脉注射至C57BL小鼠体内,观察其成像能力。结果筛选到稳定表达荧光素酶的小鼠MSCs系OP9 luc+;活性测定证明其表达效率高;在动物体内可以清晰成像。结论构建了稳定高表达荧光素酶基因的小鼠MSCs系OP9 luc+。Objective To establish murine bone marrow mesenchymal stem cell lines that can stably express the luciferase. Methods OP9 cells of MSC lines were infected by lentivirus GV260 containing luciferase gene and screened by puromycin for establishing OP9 luc＋ cell lines that could stably express the lucfferase. The activity of reporter gene of luciferase was detected and its expression effect was observed. OP9 luc ＋ cells were injected via the tail vein of C57BL mice and imaging ability was observed. Results OP9 luc＋ cells that stably expressed luciferase were screened. The activity detection proved that the expression efficiency of OP9 luc ＋ ceils was high. OP9 luc ＋ cells could form image dearly in vivo. Conclusion Murine bone marrow mesenchymal stem cell fines with stable and high expression of luciferase are established.

关 键 词：荧光素酶 活体成像 生物发光 骨髓间充质干细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]