幽门螺杆菌重组热休克蛋白60对U937细胞分泌TNF-α和IL-17的影响  

Effects of helicobacter pylori recombinant heat shock protein 60 on the secretion of TNF-α and IL-17 after stimulating U937 cells

作　　者：张珍[1] 魏义花[2] 杜镇镇[1] 周秀芝[1] 

机构地区：[1]滨州医学院病原生物学实验室,山东滨州256603 [2]滨州医学院临床学院,山东滨州256603

出　　处：《现代医药卫生》2015年第1期14-15,18,共3页Journal of Modern Medicine & Health

基　　金：山东省医药卫生科技发展计划项目(2011HZ004);山东省高等学校科技计划项目(J11LF73)

摘　　要：目的观察幽门螺杆菌重组热休克蛋白60对U937细胞分泌TNF-α与IL-17的影响。方法将由基因定点突变、重组后表达的胃癌来源株的幽门螺杆菌重组热休克蛋白60(rhsp60,即rhsp60组)及非胃癌来源株热休克蛋白60(hsp60,即hsp60组)分别与经佛波酯(PMA)诱导分化的U937细胞共培养,调整各组蛋白最终浓度分别为50、100、200μg/m L,并在12、18、24 h时收集上清液,ELISA检测上清液中TNF-α、IL-17水平,设磷酸盐缓冲液对照组。结果rhsp60组与hsp60组TNF-α、IL-17分泌水平均明显高于对照组,且同时间点、同浓度下rhsp60组明显高于hsp60组,差异均有统计学意义(P<0.05)。结论 rhsp60与hsp60均可促进U937细胞对细胞因子TNF-α、IL-17的分泌,且胃癌来源的rhsp60使TNF-α、IL-17分泌量更多。Objective To investigate the effects of helicobacter pylori recombinant heat shock protein 60 on the cell se-cretion of TNF-αand IL-17 of U937 cells. Methods Hsp60 and rhsp60 were expressed by gene mutation and recombination,which were co-cultured with 50,100,200 μg/m L of rhsp60（rhsp60 group） and hsp60（hsp60 group） with U937 cells induced by phorbol myristate acetate（PMA）. The final concentration of the groups was adjusted as 50 μg/m L,100 μg/m L,200 μg/m L. The supernatant was collected at 12 ^th,18^th,24^ thh and IL-17,TNF-α secretion was measured by ELISA. The experiment was setted the phosphate buffer as the control group. Results The TNF-α,IL-17 secretion concentrations in the hsp60 and rhsp60 groups were significantly higher than those in the control group. The TNF-α,IL-17 secretion concentrations in rhsp60 group was higher than that in hsp60 group at the same time and the same concentration. The difference had statistical significance（P〈0.05）. Conclusion The rhsp60 and hsp60 could promote the secretion of IL-17,TNF-α and rhsp60 derived from gastric cancer had more effects than hsp60 after stimulating U937 cells. secretory cell factor TNF-α and IL-17 of U937 cells.

关 键 词：螺杆菌 幽门 监控蛋白60 肿瘤坏死因子α 白细胞介素17 重组 遗传 U937细胞 基因 突变 

分 类 号：R378.99[医药卫生—病原生物学]