人参皂苷衍生物AD-1对肺癌A549细胞增殖和荷瘤裸小鼠肿瘤生长的抑制作用研究  

Study on the Inhibiting Effect of Ginseng Saponin Derivative AD-1 on Lung Cancer A549 Cell roliferation and Tumor-Bearing Nude Mice Tumor Growth

作　　者：龙浩[1] 

机构地区：[1]重庆市肿瘤研究所,重庆400030

出　　处：《中国药业》2015年第1期25-27,共3页China Pharmaceuticals

摘　　要：目的研究人参皂苷衍生物AD-1对小细胞肺癌A549细胞增殖和荷瘤裸小鼠肿瘤生长的抑制作用。方法采用噻唑蓝(MTT)法观察AD-1对小细胞肺癌A549细胞增殖的抑制作用,流式细胞术测定AD-1对小细胞肺癌A549细胞凋亡及周期的影响,并在裸小鼠人肺癌模型上观察AD-1对荷瘤裸小鼠肿瘤生长的抑制作用。结果 AD-1对A549细胞具有明显的抑制作用并呈剂量和时间依赖性;流式细胞术结果显示,细胞周期被阻滞在G0/G1期,24 h凋亡率分别为(26.37±2.62)%,(29.68±2.59)%和(35.38±2.62)%;荷瘤裸小鼠动物抑瘤试验表明,AD-1对裸小鼠的肿瘤生长也具有明显的抑制作用,抑瘤率分别为19.69%,36.53%和52.28%。结论AD-1对A549细胞的增殖和荷瘤裸小鼠肿瘤的生长具有明显的抑制作用。Objective To study the inhibiting effects of Ginseng saponin derivative AD-1 on the proliferation of human lung carcinoma A549 cell line and the tumor growth of tumor-bearing nude mice. Methods The human lung carcinoma A549 cell line was treated with AD-1. The cell grow rate was determined with the MTT assay. The effects of AD-1 on the apoptosis rate and cell cycle were observed by the flow cytometry. The subcutaneous transplantable tumor model of human lung cancer in nude mice was established. The tumor inhibitory rate was calculated to evaluate the anti-tumor effect. Results AD-1 had obvious inhibiting effect on the proliferation of human lung carcinoma A549 cells in a time-and dose-dependent manner. The flow cytometry results indicated that the cell cycle was arrested at the G0 / G1 phase. The apoptosis rates in 24 h were（ 26. 37 ± 2. 62） %,（ 29. 68 ± 2. 59） % and（ 35. 38 ± 2. 62） %,respectively. The tumor-bearing nude mice animal experiment revealed that AD-1 had the obvious inhibiting effect on the tumor growth in nude mice with the tumor inhibitory rate of 19. 69%,36. 53% and 52. 28% respectively. Conclusion AD-1 could apparently inhibit the proliferation of lung cancer A549 cell and the tumor growth of nude mice.

关 键 词：人参皂苷衍生物 肿瘤细胞 肺癌 裸小鼠 

分 类 号：R285.5[医药卫生—中药学] R282.71[医药卫生—中医学]