穿山龙总皂苷对慢性迁延期哮喘小鼠白介素-17A表达的影响  

Influence of Con-saponin of Dioscoreae Nipponicae on Interleukin-17A in Chronic Asthmatic Mice

作　　者：叶育双 宋康[2] 江立斌[2] 

机构地区：[1]杭州市江干区采荷街道社区卫生服务中心,浙江杭州310020 [2]浙江省中医院,浙江杭州310006

出　　处：《中华中医药学刊》2015年第1期168-171,I0020,I0021,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科学研究基金计划项目(2011ZB035)

摘　　要：目的：研究穿山龙总皂苷对抑制慢性迁延期哮喘小鼠气道壁及支气管平滑肌增生,抑制肺泡灌洗液、肺组织匀浆中白介素-17A的表达的影响。方法：清洁级BALB/c小鼠70只随机分为空白对照组、模型对照组、穿山龙总皂苷高剂量组、中剂量组、低剂量组、阳性对照组（醋酸泼尼松组）、穿山龙总皂苷＋醋酸泼尼松组。卵蛋白（OVA）致敏激发小鼠制作哮喘小鼠模型,在实验第18~55天,空白对照组、模型对照组以生理盐水灌胃,治疗组穿山龙总皂苷高、中、低剂量组分别每天灌胃给予穿山龙总皂苷80、40、20 mg/kg,阳性对照组给醋酸泼尼松混悬液10 mg/kg,穿山龙总皂苷＋醋酸泼尼松组给穿山龙总皂苷40 mg/kg和醋酸泼尼松5 mg/kg。实验结束后HE染色观察哮喘小鼠肺组织病理形态变化,测定气道平滑肌面积和气道内壁面积,ELISA法测定肺泡灌洗液和肺组织匀浆中白介素-17A水平。结果：穿山龙总皂苷能抑制慢性迁延期哮喘小鼠气道壁及支气管平滑肌的增生,抑制其肺泡灌洗液和肺匀浆中白介素-17A表达的水平。与空白对照组比较,模型对照组小鼠气道平滑肌、气道内壁增厚（P〈0.01）,肺泡灌洗液和肺组织匀浆中IL-17A明显升高（P〈0.01）;与模型对照组比较,气道平滑肌、气道内壁增厚缓解（P〈0.01）,低、高、中剂量组IL-17A水平降低（P〈0.01）;穿山龙治疗组间比较,中、高剂量组比低剂量组对气道平滑肌、气道内壁增厚改善效果明显（P均〈0.05）,中剂量组对小鼠IL-17A表达的影响更为显著（P〈0.01）;与阳性对照组比较,穿山龙总皂苷＋醋酸泼尼松组对小鼠IL-17A表达影响更为显著（P〈0.01）。结论：穿山龙总皂苷能够抑制慢性迁延期哮喘小鼠气道壁及支气管平滑肌的增生,抑制其肺泡灌洗液、肺组织匀浆中白介素-17A的表达,控制哮喘的发生、发展。Objective：To study the influence of Con- saponin of Dioscoreae Nipponicae（CDN） on the suppression of chronic asthma and bronchial aim.ay wall smooth muscle proliferation and inhibition of alveolar lavage fluid and the ex- pression of interleukin- 17A（ IL- 17A） in lung tissue. Method：A total of 70 clean- level BALB/c mice were randomly divided into normal control group, model control group, high - dose CDN group, medium - dose CDN group, low - dose CDN group,positive control group （prednisone group） and CDN ＋ prednisoue group. The seven groups except normal group were induced as asthmatic modeling allergies mice with ovalbumin （OVA）. During day 18 -55 ,the normal control group and model control group were treated with saline by intragastric administration. Meanwhile, the treatment groups and the high,medium,low - dose groups were treated daily with 80 mg/kg,40 mg/kg and 20 mg/kg of CDN respectively. The positive control group was given l0 mg/kg of prednisone suspension. The CDN ＋ prednisone group was given 40 mg/ kg of CDN and 5 mg/kg prednisone. HE staining was used to observe the experiment asthma pathological changes in the lung tissue of mice and we determined the airway wall area and airway smooth muscle area. ELISA assay was used to ob- serve the lavage fluid and lung tissue interleukin - 17A levels. Result：CDN improved airway wall thickening asthma andbronchial smooth muscle and inhibited the expressions of IL - 17A in the alveolar lavage fluid and lung tissue homoge- nates of chronic asthmatic mice. Compared to the normal control group, airway smooth muscle and airway wall thickened in the model control group （P 〈0.01 ） and the expression levels of IL - 17A in the alveolar lavage fluid and lung tissue homogenates of the mice in the model control group were significantly higher （ P 〈 0.01 ）. The treatment groups （ high, medium,low- dose groups） showed airway smooth muscle and airway wall thickening relieved in the model control group （P〈0.01） and IL- 17A level

关 键 词：穿山龙总皂苷 支气管哮喘 白介素-17A 小鼠 

分 类 号：R285.5[医药卫生—中药学]