首乌益智胶囊对血管性认知障碍大鼠脑组织超微结构的影响  

Effect of Shouwuyizhi capsule on the ultrastructure of brain tissue vascular dementia model rats

作　　者：杨晓妮[1] 李长生[1] 陈超[1] 程广清[1] 

机构地区：[1]山东省千佛山医院中医科,济南250014

出　　处：《中国医药》2015年第1期82-84,共3页China Medicine

基　　金：国家自然科学基金（81302940、30873323）;山东省中医药科技发展计划（2011-192）

摘　　要：目的 探讨中药首乌益智胶囊对血管性认知障碍模型大鼠脑组织海马神经元超微结构的影响.方法 血管性认知障碍大鼠模型制作采用右侧大脑中动脉栓塞再灌注法,造模成功后将大鼠随机分为首乌益智组（给予首乌益智胶囊混悬液灌胃,5.18 g/100 g）、脑复康组（给予脑复康混悬液灌胃,3.24 g/100 g）、模型组（给予0.9％氯化钠注射液灌胃,1 ml/100 g）,每组10只,另设假手术组10只,共40只大鼠.药物干预28 d后,采用透镜电镜观察各组大鼠海马区神经元的超微结构.结果 假手术组10只大鼠海马神经元细胞核清晰可辨,核内细胞器丰富且结构清晰;模型组9只大鼠海马锥体细胞明显缺失、线粒体肿胀明显、血管周围水肿;脑复康组7只大鼠海马区神经细胞核膜结构较清晰,部分线粒体絮状变,髓鞘结构较清晰,与模型组相比明显改善;首乌益智胶囊组9只大鼠细胞器基本正常.结论 首乌益智胶囊能明显改善VCI大鼠神经元的损伤.Objective To investigate the effect of Shouwuyizhi （SWYZ） capsule on the ultratructure of brain tissue in experimental rats with vascular cognitive impairment （VCI）.Methods The VCI model rats were established with MCAO method.All experimental rats were divided into Shouwuyizhi group,Naofukang group,model group and sham operation group.After 4 weeks,the rats cerebral histomorphological change was observed.Results In the sham operation group,the nucleus of hippocampal neurons was clear,organelles were abundant and had clear nuclei structure in 10 rats.In the model group,hippocampal pyramidal cells were missed with swollen mitochondria and perivascular edema in 9 rats.In the Naofukang group,hippocampus nerve cells＇ membrane structure was relatively clear; some mitochondria presented flocculent degeneration and myelin structure was clear in 7 rats.In the Shouwuyizhi group,the loss of pyramidal cells was reduced; organelles were basically normal; perivascular edema was significantly relieved in 9 rats.Conclusion SWYZ capsule can significantly improve the VCI rat neuronal injury.

关 键 词：血管性认知障碍 首乌益智胶囊 动物实验 

分 类 号：R277.7[医药卫生—中医学]