肝肾阴虚型外阴硬化性苔癣与杀伤细胞免疫球蛋白受体基因多态性的相关研究  

Association study between vulvar lichen sclerosus of the liver and kidney yin-deficiency type and polymorphisms of killer cell immunoglobulin-like recepter gene

作　　者：刘桂兰 曹峰林[2] 赵铭宇 石晶 刘树辉 

机构地区：[1]黑龙江省中医药科学院妇科,哈尔滨150036 [2]哈尔滨医科大学附属第一医院细胞生物治疗中心

出　　处：《中国医药》2015年第1期122-125,共4页China Medicine

基　　金：哈尔滨市科学技术局科技创新人才研究专项资金项目（2010RFXYS051）

摘　　要：目的 探讨肝肾阴虚型外阴硬化性苔癣（VLS）与杀伤细胞免疫球蛋白样受体（KIR）基因多态性的相关性.方法 选择30例肝肾阴虚型外阴硬化性苔癣患者作为试验组,30例体检健康女性为对照组.取受试者外周静脉血提取出人类基因组DNA,采用序列特异性引物聚合酶链反应（PCR-SSP）的方法,扩增出KIR基因的14个基因型.结果 30例VLS患者和30例健康对照个体中共检出了14个KIR基因,除框架基因KIR2DL4、KIR3DL2和KIR3DL3之外,KIR3DL1、KIR2DL3基因的表现型频率也是100％,KIR2DL1基因的表现型频率大于90％;其余依次为KIR2DL5、KIR2DS2、KIR2DS4、KIR2DS5的表现型频率在30％ ～ 70％之间;表现型频率较低者为KIR2DL2、KIR2DS1、KIR2DS3、KIR3DS1,分别为6.7％、0.0％、3.3％、13.3％.其中,实验组KIR2DL2基因和KIR3DS1基因与对照组比较差异有统计学意义（P＜0.01）,其基因频率分别是0.03和0.07,而其他12个基因与对照组比较差异均无统计学意义（P＞0.05）.结论 KIR基因多态性与肝肾阴虚型VLS的发病有一定的相关性,抑制性基因KIR2DL2和激活性基因KIR3DS1可能是本病的拮抗性基因,其差异性表达可能在肝肾阴虚型VLS的发病发挥作用.Objective To study the correlation among vulvar lichen sclerosus（VLS）,liver and kidneyyindeficiency type and killer cell immunoglobulin-like receptor （KIR） gene polymorphism.Methods Totally 30 patients with liver and kidney indeficiency were as experimental group and the 30 healthy women were as control group.Mter the human genomic DNA of human peripheral blood leukocytes was tested; 14 polymorphisms of KIR gene were amplified by sequence-specific primer polymerase chain reaction （PCR-SSP） method.Results We detected all the 14 KIR gene polymorphisms in 30 VLS cases and 30 controls.Besides frame gene,phenotype frequency of KIR2DL3,KIR3DL1 gene was also 100％ ; phenotype frequency of KIR2DLl was ＞90％,followed by KIR2DL5,KIR2DS2,KIR2DS4,KIR2DS5 phenotype frequencies.Among them,KIR2DL2 and KIR3DS1 had significant differences between twogroups（P ＜0.01） ; the gene frequencies were 0.03 and 0.07.There were no significant difference amongother 12 genes （P ＞ 0.05）.Conclusions There is a correlation between KIR gene polymorphisms and the onset of the liver and kidney yin deficiency type VLS＇ inhibitory gene KIR2DL2 ;the activated gene KIR3DS1 may be the antagonistic gene of VLS.The gene differential expression may play a role in the pathogenesis of liver and kidney yin deficiency type VLS.

关 键 词：外阴硬化性苔癣 杀伤细胞免疫球蛋白受体基因 多态性 

分 类 号：R725[医药卫生—儿科]