荆花胃康胶丸对幽门螺杆菌感染小鼠胃黏膜核因子κB p65表达的影响  

Effects of Jinghua Weikang Capsule on NF-κB p65 Expression in Gastric Mucosa of Helicobacter pylori Infected Mice

作　　者：叶晖[1] 李宁[2] 于靖[2] 成虹[2] 张学智[2] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]北京大学第一医院,北京100034

出　　处：《中国中医药信息杂志》2015年第2期60-63,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81072952)

摘　　要：目的观察荆花胃康胶丸对幽门螺杆菌(Hp)感染小鼠胃黏膜核因子κB(NF-κB)p65表达的影响,探讨其抗炎作用机制。方法将44只SPF级KM小鼠随机分为空白组、模型组、三联组、荆花胃康组。除空白组外,其他小鼠采用短期免疫抑制联合经口感染方法建立Hp感染模型,三联组予兰索拉唑、甲硝唑、克拉霉素1周,荆花胃康组予荆花胃康胶丸挥发油4周,模型组给予灭菌注射用水,空白组不予干预。所有小鼠均于给药4周后处死。通过实时荧光定量PCR、免疫组化法测定NF-κB p65的表达。结果 NF-κB p65 m RNA相对表达量为空白组3.27±0.53、模型组2.18±0.50、三联组4.19±1.21、荆花胃康组7.70±1.41(P<0.001),与模型组比较,其他3组表达均增高(P=0.004),三联组与荆花胃康组比较差异有统计学意义(P<0.001)。免疫组化显示,NF-κB p65细胞核平均光密度值为空白组25.18±7.42、模型组31.17±2.98、三联组30.06±4.69、荆花胃康组26.94±5.19(χ2=10.960,P=0.012);细胞浆平均光密度值各组差异无统计学意义(F=1.922,P=0.134);核/浆比值为空白组0.23±0.06、模型组0.28±0.03、三联组0.26±0.04、荆花胃康组0.24±0.04(χ2=14.527,P=0.002),其中模型组较空白组明显增加(P=0.002),三联组与模型组比较差异无统计学意义(P=0.420),荆花胃康组较模型组明显降低(P=0.022),且与空白组比较差异无统计学意义(P=0.750)。结论荆花胃康胶丸通过降低Hp感染小鼠NF-κB p65表达水平达到抗炎作用。Objective To observe the effects of Jinghua Weikang Capsule on NF-κB p65 expression in gastric mucosa of Helicobacter pylori(Hp) infected mice;To discuss its mechanism of action. Methods Totally 44 SPF KM mice were randomly divided into blank group, model group, triple therapy group and Jinghua Weikang group. Except for the blank group, Hp infected model was set up by short term immunosuppression combined with peroral infection. The treatment strategies were triple therapy group with lansoprazole, metronidazole and clarithromycin for 1 week, Jinghua Weikang group with Jinghua Weikang volatile oil for 4 weeks, model group with sterile water for 4 weeks, and blank group free of any treatments. All the subjects were sacrificed in the 4th week after the treatment. NF-κB p65 was detected through real-time PCR and immunohistochemical method. Results The relative quantitative expressions of NF-κB p65 m RNA were 3.27±0.53 in blank group, 2.18±0.50 in control group, 4.19±1.21 in triple therapy group, and 7.70±1.41 in Jinghua Weikang group(χ2=86.303, P <0.001). Compared with model group, the expressions of NF-κB p65 m RNA of other three groups increased statistically differently(P =0.004), and there was also statistical difference between triple therapy and Jinghua Weikang group. Through immunohistochemical tests, the values of NF-κB p65 mean optical density of nucleus were 25.18±7.42 in blank group, 31.17±2.98 in model group, 30.06±4.69 in triple therapy group, and 26.94±5.19 in Jinghua Weikang group(χ2=10.960, P =0.012). The values of NF-κB p65 mean optical density of cytoplasm had no statistical difference(F =1.922, P =0.134), and the nucleus/ cytoplasm values were 0.23±0.06 in blank group, 0.28±0.03 in model group, 0.26±0.04 in triple therapy group, and 0.24±0.04 in Jinghua Weikang group(χ2=14.527, P =0.002). The statistical analysis of the nucleus/cytoplasm values showed significance between blank and model group(P =0.002), while triple therapy group was still equal to model group(P =0.420). Jingh

关 键 词：荆花胃康胶丸 幽门螺杆菌 核因子ΚB 小鼠 

分 类 号：R285.5[医药卫生—中药学]