灭幽汤对幽门螺杆菌相关性胃炎脾胃湿热证模型小鼠Toll样受体2、4的影响  

Effects of Mieyou Decoction on TLR2 and TLR4 in H. pylori-associated Gastritis Mice of Spleen-stomach Damp-heat Syndrome

作　　者：王小娟 罗燕[2] 喻斌 徐寅 郭璇[2] 杜中华[2] 尹姣[2] 夏蓉[2] 

机构地区：[1]湖南中医大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410007

出　　处：《中国中医药信息杂志》2015年第2期64-67,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：湖南省自然科学基金(12JJ2046);湖南省科技厅资助项目(2011SK3096);湖南省中医药科研计划项目(2014165);湖南省科普计划项目(2013KP0111)

摘　　要：目的探讨灭幽汤治疗幽门螺杆菌(Hp)相关性胃炎脾胃湿热证的作用机制。方法将70只BALB/c小鼠随机分为对照组、模型组、胃三联组和灭幽汤高、低剂量组(中药高、低剂量组),每组14只,采用复合因素建立BALB/c小鼠Hp相关性胃炎脾胃湿热证模型。成模后各给药组给予相应药物,连续14 d,采用免疫组化检测Toll样受体(TLR)2、TLR4蛋白表达,实时荧光定量PCR检测TLR2、TLR4基因表达,ELISA检测血清白细胞介素(IL)-6、IL-8的表达。结果模型组TLR2、TLR4蛋白和基因表达水平及血清IL-6、IL-8含量均高于对照组(P<0.01);中药高、低剂量组和胃三联组TLR2、TLR4蛋白和基因表达及血清IL-6、IL-8含量均低于模型组(P<0.01);中药高剂量组TLR2、TLR4蛋白和基因表达水平及血清IL-6、IL-8含量虽低于胃三联组,但差异无统计学意义(P>0.05)。结论灭幽汤可能通过抑制TLR2、TLR4及下游炎症因子的表达达到治疗Hp相关性胃炎脾胃湿热证的目的。Objective To investigate the mechanisms of Mieyou Decoction in the treatment of Hp-associated gastritis of spleen-stomach damp-heat syndrome. Methods Seventy BALB/c mice were randomly divided into control group, model group, high concentration of Mieyou Decoction group, low concentrations of Mieyou Decoction group, and gastric triad group, with 14 rats in each group. BALB/c mice of Hp-associated gastritis of spleen-stomach damp-heat syndrome models were established by composite factor. After the success of modeling and continuous 14-day administration, the expressions of TLR2, TLR4 protein, TLR2 mRNA, and TLR4 mRNA were detected by immunohistochemistry and qPCR. ELISA was used to detect the expressions of IL-6 and IL-8 in the serum. Results Compared with control group, the expressions of TLR2, TLR4 protein, mRNA, IL-6, and IL-8 in model group significantly increased (P<0.01);the expressions of TLR2, TLR4 protein, mRNA, IL-6, and IL-8 in high concentration of Mieyou Decoction group, low concentrations of Mieyou Decoction group, gastric triad group were lower than model group, with statistical significance (P<0.01);the expressions of TLR2, TLR4 protein, mRNA, IL-6, and IL-8 in high concentration of Mieyou Decoction group were higher than gastric triad group, but the differences were not statistically significant (P>0.05). Conclusion Mieyou Decoction may play a role in the treatment of Hp-associated gastritis of spleen-stomach damp-heat syndrome through intervention in the expressions of TLR2 and TLR4.

关 键 词：灭幽汤 幽门螺杆菌相关性胃炎 脾胃湿热证 TOLL样受体2 TOLL样受体4 小鼠 

分 类 号：R285.5[医药卫生—中药学]