不同黄芪和党参栽培品种(系)遗传关系的ISSR分析  

Analysis of Genetic Relationship of Astragalus membranaceus and Codonopsis pilosula from Different Cultivars(Varieties) by ISSR

作　　者：朱田田[1,2,3] 晋玲[1,2] 刘效瑞 杜弢[1,3] 李应东[1] 张裴斯[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000 [2]甘肃中医学院中(藏)药资源研究所,甘肃兰州730000 [3]甘肃中医学院药用植物遗传育种研究所,甘肃兰州730000 [4]定西市农业科学研究院,甘肃定西743000

出　　处：《中国中医药信息杂志》2015年第2期79-82,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家科技支撑计划(2011BAI05B02);国家自然科学基金(81360615);甘肃省自然科学基金(1308RJZA127)

摘　　要：目的研究不同品种(系)黄芪和党参的遗传关系。方法对25个不同品种(系)黄芪和17个不同品种(系)党参进行ISSR-PCR扩增,用Popgen32软件分析其遗传多样性及遗传距离,应用NTSYS软件构建Nei's遗传距离UPGMA聚类。结果黄芪、党参样本分别检测出62、100个位点,多态位点百分率均为100%,Shannon信息指数平均值分别为0.537 6、0.472 7,Nei's基因多样度平均值分别为0.361 3、0.307 4;黄芪在遗传距离0.59、党参在遗传距离0.50处均被聚为2类。结论不同品种(系)的黄芪和党参在物种水平上具有较高的遗传多样性,且品种(系)间遗传差异较大。Objective To investigate the genetic relationships of Astragalus membranaceus and Codonopsis pilosula from the different cultivars(varieties). Methods Twenty-five samples of A. membranaceus and seventeen samples of C. Pilosula were amplified by ISSR-PCR. Genetic diversity and genetic distance were analyzed by Popgen32. UPGMA dendrogram relationship was clustered by NTSYS. Results Totally 62 and 100 loci were detected respectively from A. membranaceus and C. pilosula. All percentages of polymorphic loci(PPL) were 100%. Shannon's information indexes(I) were 0.537 6 and 0.472 7. Nei's genetic diversity indexes(H) were 0.361 3 and 0.307 4. A. membranaceus was divided into two groups in genetic distance 0.59, and C. pilosula was alike in 0.50. Conclusion Different cultivars(varieties) of A. membranaceus and C. pilosula had rich genetic diversities among species, and had large genetic differences.

关 键 词：黄芪 党参 ISSR分子标记技术 遗传关系 

分 类 号：R282.5[医药卫生—中药学]