二次正交旋转组合设计法优化蛇床子素羟丙基-β-环糊精包合工艺  

Optimization of Inclusion Compound for Osthol with Hydroxypropyl-β-Cyclodextrin by Orthogonal and Rotational Combination Design

作　　者：王锐[1] 闫丽莉[1] 谢国梁[1] 杨婧[1] 刘焱南[1] 王艳宏[1] 李伟男[1] 管庆霞[1] 李永吉[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中国中医药信息杂志》2015年第2期83-85,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：黑龙江省教育厅科学技术研究项目(12531629)

摘　　要：目的确定蛇床子素羟丙基-β-环糊精包合工艺。方法采用不饱和水溶液-冷冻干燥法制备包合物,以二次正交旋转组合设计法设计羟丙基-β-环糊精包合工艺,采用高效液相色谱法测定包封率。结果确定最佳包合工艺为:羟丙基-β-环糊精与药物比例为4.5∶1,电动搅拌机35℃,恒温水浴搅拌210 min,进行包封。结论所确定的制备工艺合理可行,具有良好的开发应用前景。Objective To study the process of hydroxypropyl-β-cyclodextrin （HP-β-CD） inclusiontechniques for osthol. Methods The inclusion complex of osthol and HP-β-CD was prepared byunsaturated water solution and freeze-drying technique. Inclusion techniques were selected byscreening on quadratic orthogonal rotation combination design method, and the entrapmentefficiency was identified by HPLC. Results The optimal technical conditions were as follows：theratio of HP-β-CD and drug was 4.5∶1;temperature for electric mixer was 35 ℃;the stirring timefor thermostatic waterbath was 210 min. Conclusion This method is reasonable and it may have aprosperous future of development and application.

关 键 词：蛇床子素 包合工艺 羟丙基-Β-环糊精 二次正交旋转组合设计 

分 类 号：R283.5[医药卫生—中药学]