早期糖尿病肾病肾阳虚证的血浆蛋白质组学分析  

Plasma Proteomic Analysis of Early Diabetic Nephropathy Patients with Kidney Yang Deficiency

作　　者：卢洪梅[1] 汤水福[2] 胡坤华[3] 

机构地区：[1]广东医学院第二临床医学院中医学教研室,广东东莞523808 [2]广州中医药大学第一附属医院肾内科,广东广州510405 [3]中山医科大学基础医学院蛋白质组学教研室,广东广州510000

出　　处：《广州中医药大学学报》2014年第6期890-894,1027,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东医学院科研基金博士学位人员科研启动项目(编号:B2013008)

摘　　要：【目的】寻找糖尿病肾病肾阳虚证敏感的血浆分子标志物。【方法】对4例早期糖尿病肾病肾阳虚证患者的血浆和4例正常人血浆进行荧光差异双向电泳(2-D DIGE)分析,选择表达差异大于1.5倍的蛋白斑点进行质谱分析。【结果】成功建立糖尿病肾病肾阳虚证血浆和正常血浆的胶内差异双向凝胶电泳图谱,通过分析共鉴定出9种差异蛋白质,包括补体C3、补体C4、载脂蛋白E、泛素化因子等。【结论】荧光差异双向电泳能够客观地显示糖尿病肾病肾阳虚证血浆与正常血浆之间的蛋白质表达差异,经鉴定的9种蛋白有可能为糖尿病肾病的早期诊断以及中医证型研究提供潜在的血浆分子标志物。Objective To screen the sensitive plasma molecular markers in diabetic nephropathy （ DN） patients with kidney yang deficiency. Methods Two-dimensional fluorescence difference gel electrophoresis （2-D DIGE） was used to analyze the plasma which was sampled from 4 early DN patients with kidney yang deficiency and 4 healthy adult volunteers. Proteins that showed differential expression with an over 1.5-fold change were analyzed by MALDI-TOF/TOF mass spectrometry. Results Two-dimensional electrophoresis （2-DE） maps of plasma proteins in DN patients with kidney yang deficiency and healthy adults were established successfully. We validated 9 differentially expressed proteins, including complements C3 and C4, apolipoprotein E and ubiquitination factor. Conclusion Proteomic analysis by 2-D DIGE can objectively reveal the differences of plasma protein expression in DN patents with kidney yang deficiency and healthy adult volunteers. The obtained 9 proteins have potential to provide plasma molecular markers for the early clinical diagnosis and for the research of traditional Chinese medical patterns of DN.

关 键 词：糖尿病肾病 肾阳虚证 蛋白质组学 荧光差异双向电泳 

分 类 号：R587.2[医药卫生—内分泌]