蒲黄总黄酮对2型糖尿病大鼠炎症因子和胰岛素敏感性的影响  

Effects of Pollen Typhae Total Flavone on Inflammatory Factors and Insulin Sensitivity in Type 2 Diabetic Rats

作　　者：冯晓桃[1] 陈群[1] 梁霄[1] 

机构地区：[1]广西中医药大学广西中医药科学实验中心,广西南宁530001

出　　处：《广州中医药大学学报》2014年第6期936-939,943,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81202677);广西自然科学基金资助项目(编号:2012GXNSFBA053103);广西中医基础研究重点实验室开放课题(编号:14-045-42)

摘　　要：【目的】观察活血化瘀中药蒲黄提取物蒲黄总黄酮(PTF)对2型糖尿病大鼠炎症因子和胰岛素敏感性的影响。【方法】选用SD大鼠,采用高脂饮食+小剂量链脲佐菌素诱导2型糖尿病大鼠模型,随机分为模型组、PTF组(剂量为200 mg·kg-1·d-1)和罗格列酮组(剂量为4 mg·kg-1·d-1),并设立对照组。干预治疗4周后,检测血浆白细胞介素(IL-6)、肿瘤坏死因子-α(TNF-α),并行胰岛素耐量试验,分析骨骼肌组织细胞因子信号转导抑制因子-3(SOCS-3)蛋白。【结果】治疗4周后,与对照组比较,模型组大鼠血浆IL-6、TNF-α和胰岛素抵抗指数(HOMA-IR)以及骨骼肌组织SOCS-3水平均显著升高(P﹤0.05),胰岛素耐量试验受损(P﹤0.05);与模型组比较,PTF组大鼠血浆IL-6和HOMA-IR水平均显著降低(P﹤0.05),胰岛素耐量试验受损得到显著改善(P﹤0.05),骨骼肌组织SOCS-3水平均显著低于模型组和罗格列酮组(P﹤0.05)。【结论】PTF能降低2型糖尿病大鼠血浆IL-6和骨骼肌组织SOCS-3水平,改善其胰岛素敏感性。Objective To investigate the effects of Pollen Typhae total flavone （ PTF） , an extract from Pollen Typhae which has the actions of activating blood and removing stasis, on inflammatory factors and insulin sensitivity in type 2 diabetic rats. Methods SD rats were used as the experimental animal. Type 2 diabetic rats induced by high fat diet plus low dose of streptozotocin were randomly divided into model group, PTF group （in the dosage of 200 mg·kg^-1·d^-1） , and rosiglitazone group （in the dosage of 4 mg·kg^-1·d^-1） . Additionally, normal control group was set up. After treatment for 4 weeks, plasma interleukin 6 （ IL-6） and tumor necrosis factor alpha （ TNF-α） levels were detected, the insulin tolerance test （ ITT） was performed, and the protein expression of suppressor of cytokine signaling-3 （ SOCS-3） in skeletal muscle was determined. Results After＆amp;nbsp;treatment for 4 weeks, the plasma levels of IL-6 and TNF-α, the homeostasis model of insulin resistance （ HOMA-IR） , and expression level of SOCS-3 in skeletal muscle in the model groups were significantly increased （ P﹤0.05） as compared with those in the normal control group, and insulin tolerance was also impaired in the model group （ P﹤0.05） . Compared with the model group, IL-6 level and HOMA-IR were markedly decreased in the PTF group （ P﹤0.05） , and the impaired insulin tolerance was obviously improved （P﹤0.05） . The level of SOCS-3 in the skeletal muscle of PTF group was also much lower than that of the model group and rosiglitazone group （P﹤0.05） . Conclusion PTF has effects on decreasing the levels of plasma IL-6 and SOCS-3 in the skeletal muscle and on improving insulin sensitivity in type 2 diabetic rats.

关 键 词：2型糖尿病/中药疗法 胰岛素抵抗 蒲黄总黄酮/药理学 胰岛素耐量 炎症因子 疾病模型  动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]