在无创性通气条件下快速建立小鼠心肌梗死模型的简易方法  

Efficient Establishment of Mice Myocardial Infarction Model in Non-invasive Ventilation

作　　者：卢新林[1] 岳玉霞[2] 严泉祥 何原[3] 陈文江[1] 陈灿[3] 

机构地区：[1]广东医学院,广东湛江524023 [2]河南大学附属淮河医院 [3]广东医学院附属医院

出　　处：《中国医学创新》2014年第30期23-26,共4页Medical Innovation of China

摘　　要：目的:探讨建立小鼠心肌梗死模型的快速简易方法,提高动物存活率。方法:使用戊巴比妥钠麻醉小鼠,在无创性机械通气的条件下开胸,将心脏轻轻挤出胸腔后,用手术针钩断冠状动脉的左前降支(LAD),通过观察存活率、心电图改变和组织病理学染色来评价心肌梗死模型是否成功。同时与冠状动脉结扎组进行比较。结果:LAD钩断术后心电图出现ST段抬高,TTC染色可见明显的梗死区域,术后4周心肌发生纤维化;模型组的术后存活率为85%,而冠状动脉结扎组则为54%。结论:应用这种改良的新方法可以快速并成功地建立小鼠心肌梗死模型。Objective：To discuss the method of establishing an efficient mice myocardial infarction model with a high survival rate.Method：The mice were anesthetized with pentobarbital sodium,thoracotomy in non-invasive ventilation,gently squeeze out the heart of the chest,off the hook of the left anterior descending coronary artery with operation needle,by observing the survival rate,the change of electrocardiogram,used pathology testing to prove the model to be successful. At the same time compared with the coronary artery ligation group.Result：After off the hook of the left anterior descending coronary artery,the ST-T segment of ECG raised,the infarction area was obvious with TTC staining,myocardial fiberizes 4 weeks after operation;the survival rate of myocardial infarction model was 85%,the ligation of the coronary artery group was 54%.Conclusion：Application of this new method can establish an efficient mice myocardial infarction mode.

关 键 词：心肌梗死 戊巴比妥钠 机械通气 冠状动脉结扎 

分 类 号：R542.22[医药卫生—心血管疾病] R-332[医药卫生—内科学]