大鼠弥漫性轴索损伤后免疫炎症反应的变化及氢气对其的影响  

Changes of immuno-inflammatory responses by intraperitoneal injection of hydrogen after diffuse axonal injury in rats

作　　者：马旭东[1] 宋锦宁[1] 赵永林[1] 张斌飞[1] 李丹东[1] 刘尊伟[1] 赵君杰[1] 赵雅慧[1] 

机构地区：[1]西安交通大学医学院第一附属医院神经外科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2015年第1期23-27,33,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30471774);教育部新世纪优秀人才支持计划资助项目(No.NCET-05-0831);陕西省自然科学基金资助项目(No.2003C1-16)~~

摘　　要：目的探讨免疫炎症反应在大鼠弥漫性轴索损伤(DAI)中的作用及氢气对其保护作用。方法将96只SD雄性大鼠随机平均分为正常组、假手术组、DAI模型组(6h、1d、3d)和氢气干预组(6h、1d、3d)。采用头颅瞬间旋转损伤装置制作大鼠DAI模型,干预组每日2次腹腔注射高纯度氢气(10mL/kg)。各组于预定时间点通过HE染色、嗜银染色观察组织形态学的变化,GFAP免疫荧光染色观察胶质细胞增生活化情况,Western blotting分析炎症因子IL-6、IL-1β和信号通路JNK、p-JNK蛋白的表达。结果与假手术组比较,DAI顶叶皮髓交界区出现轴索肿胀、迂曲、轴索球等DAI特征性改变,GFAP阳性细胞数量增多(P<0.05),IL-6、IL-1β和p-JNK表达增高(P<0.01),氢气干预组较模型组脑组织形态学有所改善,GFAP阳性细胞数减少(P<0.05),IL-6、IL-1β和p-JNK表达降低(P<0.01)。结论免疫炎症反应参与了DAI急性期脑损伤的病理生理过程,星形胶质细胞的活化和炎症因子的大量释放导致脑损伤的加重。氢气能够通过减轻免疫炎症反应改善DAI急性期脑损伤。减轻免疫炎症反应程度对DAI急性期脑保护具有一定意义。Objective To explore the role of immuno-inflammatory reaction in diffuse axonal injury （DA1） and the therapeutic action of hydrogen in DAI model. Methods Totally 96 adult male SD rats were divided into 8 groups （n 12 in each）： normal group, sham group, DAI groups （6 h, 1 d and 3 d） and DAI with hydrogen treatment groups（6 h, 1 d and 3 d）. Rat DAI model was induced by an experimental facility developed to make the rat head spin 90 degrees at the moment to cause shearing injury. Hydrogen gas of high purity was injected intraperitoneally to DAI rat models twice per day （10 mL/kg）. The histo-morphology, GFAP immunofluorescent staining, Western blotting （IL-6, IL-1, JNK and p-JNK） in parietal cortex were investigated at 6 h, 1 d and 3 d after DAI. Results DAI-related changes such as axon swelling and axonal ball were found in the conjunction area between DAI-affected parietal cortex and corpus callosum compared with those in sham group. The number of GFAP-positive cells as well as IL-6, IL-1, JNK and p-JNK expressions were significantly increased after DAI （ P〈0.05, P 〈 0. 01）. Hydrogen treatment could reverse the changes mentioned above. Conclusion Immuno- inflammatory reaction is involved in DAI pathophysiological process in acute phase. The release of large numbers of inflammatory factors and activation of astrocytes may lead to the deterioration of brain damage. Hydrogen can improve acute brain injury of DAI by reducing immuno-inflammatory reaction.

关 键 词：弥漫性轴索损伤 免疫炎症反应 氢气 颅脑损伤 

分 类 号：R651.15[医药卫生—外科学]