大鼠弥漫性轴索损伤后脑组织OPN分子的表达变化  

Changes of OPN expression in brain after diffuse axonal injury in rats

作　　者：黄元志 宋锦宁[1] 金涛[1] 李宇[1] 李丹东[1] 刘晓斌[1] 马旭东[1] 

机构地区：[1]西安交通大学医学院第一附属医院神经外科,陕西西安710061 [2]延安大学附属医院神经外科,陕西延安716000

出　　处：《西安交通大学学报（医学版）》2015年第1期65-69,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30471774);教育部新世纪优秀人才支持计划资助项目(No.NCET-05-0831);陕西省自然科学基金资助项目(No.2003C1-16)~~

摘　　要：目的探讨弥漫性轴索损伤(DAI)后大鼠脑组织皮层、海马以及脑干部位的骨桥蛋白(OPN)分子的表达情况及变化规律。方法 SD大鼠30只,分为对照组及损伤组(又分为3、24、48、72h和7d共5个亚组,每组5只),采用大鼠头颅瞬间旋转装置制作大鼠DAI模型;免疫组化方法对相应时间点的大鼠脑组织进行染色观察,并行免疫组化评分来检测OPN表达量的改变。结果 DAI后大鼠脑组织各部位OPN分子3h时表达就开始升高,至72h高峰后开始下降,7d时细胞外基质较其他各组染色深,结果显示OPN分子大多分泌至细胞外基质。与对照组相比,损伤组各时间点的免疫组化评分明显升高并有统计学差异(P<0.05)。OPN分子表达升高主要聚集在神经元胞体聚集的灰质以及脑干神经核团等部位,大脑以及脑干中白质、胼胝体部位胶质细胞也有少量表达。结论 OPN分子在大鼠正常脑组织中即有弱阳性表达,DAI损伤后脑组织中OPN分子的表达明显升高,它可能通过多种途径对损伤神经细胞起到保护作用。Objective To investigate the expression of osteopontin （OPN） in rat brain tissues after diffuse axonal injury （DAD. Methods We divided 30 adult male rats randomly into six groups： sham group without injury （n=5） and 5 experimental groups （3 h, 24 h, 48 h, 72 h, and 7 d post-injury, n=5 in each group）. Rat DAI model was constructed by instant head rotation. Immunohistochemical method was used to detect the expression of OPN at different time points. Images of the immunohistochemical stained sections were captured and immunohistochemical score was used to detect changes of OPN expression. Results Immunohistochemical staining showed a little OPN expression in normal rat brain. OPN expression increased rapidly at 3 h after DAI and peaked at 72 h. Stained OPN in extracellular matrix was darker compared to other groups at 7 d, showing most of OPN was secreted into the extracellular matrix. Compared with the control group, immunohistochemical scores in experimental groups at each time point were significantly higher and got statistically significant （P〈0.05）. The increased OPN mainly gathered in the gray matter based on neurons, nucleus in brainstem and other parts. There was also little OPN expression in white matter of the brain and brainstem and glial cells in corpus callosum. Conclusion OPN is weakly expressed in normal brain tissue of rats and its expression increases obviously after DIA. The increased OPN may play a protective role through a variety of pathways in the brain.

关 键 词：颅脑损伤 弥漫性轴索损伤 骨桥蛋白 脑组织 

分 类 号：R651.15[医药卫生—外科学]