大鼠弥漫性轴索损伤后Nogo-A在脑组织中表达的分布与动态变化及其意义  

Expression changes of Nogo-A and its significance in rat brain with diffusive axonal injury

作　　者：赵修[1,2] 宋锦宁[1] 郗磊[1] 隋龙[1] 王文博[1] 刘晓斌[1] 

机构地区：[1]西安交通大学医学院第一附属医院神经外科,陕西西安710061 [2]第四军医大学附属唐都医院神经外科,陕西西安710038

出　　处：《西安交通大学学报（医学版）》2015年第1期80-84,134,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30471774);教育部新世纪优秀人才支持计划资助项目(No.NCET-05-0831);陕西省自然科学基金资助项目(No.2003C1-16)~~

摘　　要：目的研究Nogo-A在弥漫性轴索损伤(DAI)大鼠脑组织中表达的分布与动态变化,探讨其在DAI后轴突再生中的作用及意义。方法 SPF级雄性SD大鼠42只,随机分为正常对照组及DAI后2h组、6h组、12h组、24h组、72h组、7d组,每组6只。应用大鼠头颅瞬间旋转损伤装置制作大鼠DAI动物模型。伤后不同时间点行病理切片HE染色及Nogo-A免疫组织化学染色。按大脑皮层、大脑白质、海马、胼胝体及脑干5个脑区,分析Nogo-A的表达变化规律。结果 DAI后各观测脑区神经元及少突胶质细胞中均可见明显的Nogo-A表达,其中以神经元表达为著,Nogo-A表达变化的时间规律为:2h即已明显升高(P<0.05),12h达高峰,至7d时,各脑区Nogo-A表达已有明显下降,但仍高于正常对照组(P<0.05)。结论 DAI后脑组织Nogo-A表达的分布与动态变化与DAI病理变化趋势基本一致。此种变化规律可能为伤后神经再生过程中在某种信号调节机制下的一种调适性表达变化,其可能为新生轴突延伸导向以及介导建立突触联系。Objective To investigate the expression changes of Nogo-A in rat brain with diffuse axonal injury （DAI） and discuss the significance and mechanisms of Nogo-A in axon regeneration after DAI. Methods Wc divided 42 male SD rats of SPF grade into 7 groups randomly： the control group, DAI 2 h group, DAI 6 h group, DA1 12 h group, DAI 24 h group, DAI 72 h group and DA1 7 d group, with 6 rats in each. The DA1 animal models were established using the rat head coronal plane moment rotation acceleration vulnerant equipment. Then HE staining and immunohistochemical staining were used respectively at several time intervals after DAI. Image collection and gradation analysis were carried out in 5 encephalic regions to analyze the changes of Nogo-A expression： the cortex, white matter, hippocampus, corpus callosum and brain stem. Results Nogo-A was expressed significantly in the neurons in all the five encephalic regions and in a small number of oligodendroglia cells, especially in the former. The expression of Nogo-A protein increased obviously 2 hours after DA1 （ P〈0.05 ）, and rcached the highest level at 12 h, and had a gradual recovery to the 2-h level by day 7. Conclusion After DAI, Nogo-A expression increased obviously and had dynamic changes, the tendency of which is the samc as the pathological change of DAI. After DAI the high expression of Nogo-A in the neurons may be rclated to thc development stage. Nogo-A may play an important role in the process of neural repair.

关 键 词：弥漫性轴索损伤 NOGO-A 中枢神经损伤 轴突再生 颅脑损伤 

分 类 号：R651.15[医药卫生—外科学]