术后疼痛大鼠切口组织Toll样受体4及其下游细胞因子表达的变化  

Expression changes of Toll-like receptor 4 and its down-stream cytokines in incisional tissues from rats with postoperative pain

作　　者：邢娜[1] 李会新[1] 王中玉[1] 柏莉莹 孔存龙[1] 卫新[1] 张卫[1] 

机构地区：[1]郑州大学第一附属医院麻醉科,450052

出　　处：《中华创伤杂志》2015年第1期86-90,共5页Chinese Journal of Trauma

摘　　要：目的 探讨术后疼痛大鼠切口组织Toll样受体4（TLR4）及其下游细胞因子IL-1β、IL-6和TNF-αmRNA表达的变化. 方法 健康成年雄性SD大鼠74只,体重200～250 g,建立大鼠术后疼痛模型.于术前1d、术后0.5,1,2,6,12h及1,2,3,5,7d测定术侧与非术侧机械缩足反射阈值（PMWT）;于术前1d、术后2,8h、1,2,3,5,7d采用荧光定量PCR法检测皮肤切口组织TLR4、IL-1β、IL-6和TNF-α mRNA的表达. 结果 与术前比较,大鼠术侧术后0.5 h～5 dPMWT降低,术后2h术侧切口组织TLR4mRNA表达下调,之后逐渐升高,IL-1β、IL-6和TNF-αmRNA于术后2,8h、1,2,3,5d表达上调（P＜0.05）,非术侧PMWT差异无统计学意义（P＞0.05）;术侧PMWT术后6h最低,之后逐渐升高,术侧切口组织TLR4mRNA术后2d表达水平最高,IL-1β、IL-6和TNF-α mRNA分别于术后2h、1d、3d表达水平最高（P＜0.05）.TLR4、IL-1β、IL-6和TNF-αmRNA表达水平与术侧PMWT呈负相关（r=-0.501,-0.743,-0.893,-0.657,P＜0.05）,IL-1β、IL-6和TNF-αmRNA与TLR4 mRNA表达水平呈正相关（r =0.764,0.283,0.667,P＜0.05）. 结论 术后疼痛大鼠切口组织TLR4及其下游炎性因子IL-1β、IL-6、TNF-α mRNA表达上调,该变化可能参与了术后疼痛的产生和维持.Objective To evaluate the changes in mRNA expressions of Toll-like receptor 4 （TLR4） and its down-stream cytokines IL-1β,IL-6 and TNF-a in incisional tissues from a rat with postoperative pain.Methods Incisional pain was induced in 74 male adult SD rats weighing 200-250 g.Paw mechanical withdrawal threshold （PMWT） around the wound on the operated and nonoperated sides was measured at 1 day before operation and at 0.5,1,2,6 and 12 hours as well as 1,2,3,5 and 7 days after operation.Skin incisional tissues were removed for determination of mRNA expressions of TLR4,IL-1β,IL-6 and TNF-a using real-time quantitative PCR at 1 day before operation and at 2 and 8 hours as well as 1,2,3,5 and 7 days after operation.Results Compared with the baseline value before operation,PMWT on the operated side was significantly decreased at 0.5 hours-5 days after operation,mRNA expression of TLR4 around the wounds on the operated side was down-regulated at 2 hours after operation followed by a gradual increase,mRNA expressions of IL-1β,IL-6 and TNF-α on the operative side were up-regulated at 2 and 8 hours as well as 1,2,3 and 5 days after operation （P 〈 0.05）,but no significant changes were found in PMWT and mRNA expressions of TLR4,IL-1β,IL-6 and TNF-α on the non-operated side（P 〉 0.05）.PMWT on the operated side was lowest at 6 hours after operation followed by the gradual increase,mRNA expression of TLR4 on the operated side peaked at 2 days after operation,and mRNA expressions of IL-1β,IL-6 and TNF-a respectively peaked at 2 hours,1 day and 3 days after operation （P 〈 0.05）.mRNA expressions of TLR4,IL-1β,IL-6 and TNF-a were negatively correlated with PMWT on the operative side （r =-0.501,-0.743,-0.893,-0.657,P 〈 0.05）,and mRNA expressions of IL-1 β,IL-6 and TNF-a were positively correlated with the level of TLR4 mRNA（r=0.764,0.283,0.667,P〈0.05）.Conclusion mRNA expressions of TLR4 and its down-stream cytokines IL-1 β,IL-6 and TNF-a in skin incisional tissues are up-regulated,which may

关 键 词：疼痛 手术后 TOLL样受体4 白细胞介素类 肿瘤坏死因子Α 

分 类 号：R285.5[医药卫生—中药学]