化学发光法检测湖北地区组织多肽抗原正常参考值范围及影响因素  

Reference Range of Tissue Polypeptide Antigen in Hubei Province People by CLIA and Its Influence Factors

作　　者：喻晶[1] 王堃[1] 雷旦生[1] 

机构地区：[1]湖北省肿瘤医院检验科,湖北武汉430079

出　　处：《武汉大学学报（医学版）》2015年第1期101-104,共4页Medical Journal of Wuhan University

摘　　要：目的:测定中国湖北地区健康人群组织多肽抗原(TPA)数据,建立正常参考值,并探讨其影响因素。方法:2013年4-10月采集144例健康成年体检者静脉血3ml,测定TPA值。再分别测定部分慢性支气管炎、肺结核、风湿病、肺心病患者血清TPA水平,以及加入干扰物胆红素、血红蛋白、甘油三酯后健康人标本的TPA水平,与正常参考值进行统计学分析。结果:144名健康人TPA正常参考值为0-129.28 U/L,与西方人参考范围0-72U/L(厂家提供的)比较,中国人群的TPA值偏高。男性与女性健康人TPA无显著性差异(P>0.05),男性组TPA为(70.32±3.51)U/L,正常参考值为0-133.87U/L;女性组TPA为(62.46±3.48)U/L,正常参考值为0-140.34U/L。我们将体检者按年龄每10岁分为一组(共分为4组),各年龄组间无统计学差异(P>0.05,表4):20-29岁组TPA为(65.23±5.46)U/L,30-39岁组TPA为(57.94±4.80)U/L,40-49岁组TPA为(71.24±5.44)U/L,50岁以上组TPA为(68.90±4.26)U/L。脂血、黄疸血、溶血标本对TPA检测的偏差均在±10%内。此外,在检测的肺部良性疾病患者中有3例慢性阻塞性肺病患者、4例肺心病患者血清TPA超出本研究参考范围。结论:建立湖北地区健康人群TPA参考范围,不受性别及年龄的影响。一些良性疾病患者血清TPA水平会假性升高,脂血、溶血、黄疸血不会影响TPA的检测水平。Objective：To determine the normal values for tissue polypeptide antigen（TPA）in Hubei Chinese healthy adult volunteers,and discuss the factors affecting its value detection.Methods：A total of 144 healthy adult volunteers were enrolled from April 2013 to October 2013.The technique was standardized with citrated blood and TPA was determined in those volunteers.Then TPA levels were also detected in patients with some benign diseases such as chronic bronchitis,pulmonary tuberculosis,and rheumatism.For interferences tests,TPA was detected in healthy specimens with bilirubin,hemoglobin and triglyceride and compared with reference value for statistical analysis.Results：The TPA reference range of 95%for 144 volunteers was 0-129.28U/L.As compared with the manufacturer＇s reference range 0-72.00U/L,TPA values of Chinesehealthy volunteers were higher.There was no significant difference of TPA reference range between different gender and age groups（P〉0.05）.The value of TPA in male group is（70.32＋3.51）U/L,and reference range is 0-133.87U/L.The value of TPA in female group is（62.46＋3.48）U/L,and reference range is 0-140.34U/L.The value of TPA in age group 20-29 years is（65.23＋5.46）U/L,in age group 30-39 years is（57.94＋4.80）U/L,in age group 40-49 years is（71.24＋5.44）U/L,and in age group 50years is（68.90＋4.26）U/L.No remarkable interference（±10%）of TPA value was found in specimens with jaundice,hemolysis,and lipid blood.In addition,TPA values of some benign diseases patients were beyond the reference range,including 3cases with chronic obstructive pulmonary disease（COPD）and 4cases of pulmonary heart disease.Conclusion：TPA reference range of people in Hubei province was established,which was not influenced by gender and age.Serum TPA levels in some benign disease patients may rise falsely.And TPA values weren＇t affected in specimens with jaundice,hemolysis,and lipid blood.

关 键 词：组织多肽抗原 参考值 影响因素 

分 类 号：R446.11[医药卫生—诊断学]